Cardiac Nerve Growth Factor Overexpression Induces Bone Marrow-derived Progenitor Cells Mobilization and Homing to the Infarcted Heart

被引：17

作者：

Meloni, Marco ^{[1
,2
]}

Cesselli, Daniela ^{[3
]}

Caporali, Andrea ^{[1
,4
]}

Mangialardi, Giuseppe ^{[1
]}

Avolio, Elisa ^{[1
,3
]}

Reni, Carlotta ^{[1
]}

Fortunato, Orazio ^{[5
]}

Martini, Stefania ^{[3
]}

Madeddu, Paolo ^{[1
]}

Valgimigli, Marco ^{[6
]}

Nikolaev, Evgeni ^{[7
]}

Kaczmarek, Leszek ^{[7
]}

Angelini, Gianni D. ^{[1
]}

Beltrami, Antonio P. ^{[3
]}

Emanueli, Costanza ^{[1
,8
]}

机构：

[1] Univ Bristol, Bristol Heart Inst, Sch Clin Sci, Bristol BS2 8HW, Avon, England

[2] Univ Glasgow, Inst Cardiovasc & Med Sci, BHF Glasgow Cardiovasc Res Ctr, Glasgow G12 8TA, Lanark, Scotland

[3] Univ Udine, Dept Med & Biol Sci, I-33100 Udine, Italy

[4] Univ Edinburgh, BHF Ctr Cardiovasc Sci, Edinburgh, Midlothian, Scotland

[5] IRCCS Multimed, Milan, Italy

[6] Univ Ferrara, Cardiol Dept, I-44100 Ferrara, Italy

[7] Nencki Inst, Lab Mol Neurobiol, Warsaw, Poland

[8] Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, London, England

来源：

MOLECULAR THERAPY | 2015年 / 23卷 / 12期

关键词：

HEMATOPOIETIC STEM-CELL; COLONY-STIMULATING FACTOR; MYOCARDIAL-INFARCTION; ACTIVATION; MIGRATION; RECRUITMENT; ANGIOGENESIS; OSTEOCLASTS; INHIBITION; EXPRESSION;

D O I：

10.1038/mt.2015.167

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Reparative response by bone marrow (BM)-derived progenitor cells (PCs) to ischemia is a multistep process that comprises the detachment from the BM endosteal niche through activation of osteoclasts and proteolytic enzymes (such as matrix metalloproteinases (MMPs)), mobilization to the circulation, and homing to the injured tissue. We previously showed that intramyocardial nerve growth factor gene transfer (NGF-GT) promotes cardiac repair following myocardial infarction (MI) in mice. Here, we investigate the impact of cardiac NGF-GT on postinfarction BM-derived PCs mobilization and homing at different time points after adenovirusmediated NGF-GT in mice. Immunohistochemistry and flow cytometry newly illustrate the temporal profile of osteoclast and activation of MMP9, PCs expansion in the BM, and liberation/homing to the injured myocardium. NGF-GT amplified these responses and increased the BM levels of active osteoclasts and MMP9, which were not observed in MMP9-deficient mice. Taken together, our results suggest a novel role for NGF in BM-derived PCs mobilization/homing following MI.

引用

页码：1854 / 1866

页数：13

共 44 条

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