A novel fluorescent probe for selective labeling of histidine

被引：43

作者：

Li, XH ^{[1
]}

Ma, HM ^{[1
]}

Nie, LH ^{[1
]}

Sun, M ^{[1
]}

Xiong, SX ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Biol Chem Lab, Beijing 100080, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 515卷 / 02期

基金：

中国国家自然科学基金;

关键词：

fluorescent probe; 1-(2,3-Epoxypropoxy)-2-phenylazo-8-acetylaminonaphthalene-3,6-disulfonic acid sodium salt; histidine labeling;

D O I：

10.1016/j.aca.2004.03.008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel water-soluble fluorescent probe, 1-(2,3-epoxypropoxy)-2-phenylazo-8-acetylamino-naphthalene-3,6-disulfonic acid sodium salt (EPANS), was designed and synthesized by reacting epichlorohydrin with azophloxine, and its properties for labeling of histidine were studied. In alkaline media, EPANS reacted selectively with histidine rather than with other amino acids, causing a large decrease in fluorescence intensity and thereby allowing a selective detection of histidine without a tedious separation. The linear range was 4-500 muM with a detection limit of 1 muM (S/N = 3). The proposed method has been successfully used to determine histidine in human serum. The obtained results were in good agreement with those given by using histidine-nickel complex adsorptive voltammetry. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：255 / 260

页数：6