Zebrafish as a model to study PTPs during development

被引：5

作者：

Overman, Jeroen Paardekooper ^{[1
,2
]}

den Hertog, Jeroen ^{[1
,2
,3
]}

机构：

[1] Hubrecht Inst KNAM, Utrecht, Netherlands

[2] Univ Med Ctr Utrecht, Utrecht, Netherlands

[3] Leiden Univ, Inst Biol Leiden, NL-2300 RA Leiden, Netherlands

来源：

METHODS | 2014年 / 65卷 / 02期

关键词：

Protein-tyrosine phosphatase; Zebrafish; Gastrulation cell movements; Phosphoproteomics; Phosphotyrosine; PROTEIN-TYROSINE PHOSPHATASES; ZINC-FINGER NUCLEASES; VERTEBRATE GASTRULATION; EMBRYONIC-DEVELOPMENT; CELL MOVEMENTS; HUMAN GENOME; GENE; FYN/YES; SYSTEM; PHOSPHOPROTEOMICS;

D O I：

10.1016/j.ymeth.2013.08.018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein-tyrosine phosphatases (PTPs) have important roles in signaling, but relatively little is known about their function in vivo. We are using the zebrafish as a model to study the function of PTPs at the organismal, cellular and molecular level. The zebrafish is an excellent experimental model for the analysis of gene function. We have developed methods to quantitatively study effects of PTP knockdown or expression of (mutant) PTPs, particularly with respect to gastrulation cell movements. Moreover, we have studied the phosphoproteome of zebrafish embryos. In this review, we will discuss methods to manipulate the zebrafish genome and techniques that we have developed to assess developmental defects during gastrulation and to assess differences in the phosphoproteome. (C) 2014 Published by Elsevier Inc.

引用

页码：247 / 253

页数：7

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