Chlorogenic Acid Inhibits Human Platelet Activation and Thrombus Formation

被引：91

作者：

Fuentes, Eduardo ^{[1
,2
]}

Caballero, Julio ^{[3
]}

Alarcon, Marcelo ^{[1
,2
]}

Rojas, Armando ^{[4
]}

Palomo, Ivan ^{[1
,2
]}

机构：

[1] Univ Talca, Dept Clin Biochem & Immunohematol, Interdisciplinary Excellence Res Program Hlth Agi, Fac Hlth Sci, Talca, Chile

[2] CONICYT Reg, CEAP, Gore Maule, Talca, Chile

[3] Univ Talca, Fac Engn Bioinformat, Ctr Bioinformat & Mol Simulat, Talca, Chile

[4] Catholic Univ Maule, Fac Med, Biomed Res Labs, Talca, Chile

来源：

PLOS ONE | 2014年 / 9卷 / 03期

关键词：

P-SELECTIN EXPRESSION; PROTEIN-KINASE-A; ADENOSINE RECEPTOR; INCREASES; THERAPY; ASPIRIN; DOCKING; BINDING; COFFEE;

D O I：

10.1371/journal.pone.0090699

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Chlorogenic acid is a potent phenolic antioxidant. However, its effect on platelet aggregation, a critical factor in arterial thrombosis, remains unclear. Consequently, chlorogenic acid-action mechanisms in preventing platelet activation and thrombus formation were examined. Methods and Results: Chlorogenic acid in a dose-dependent manner (0.1 to 1 mmol/L) inhibited platelet secretion and aggregation induced by ADP, collagen, arachidonic acid and TRAP-6, and diminished platelet firm adhesion/aggregation and platelet-leukocyte interactions under flow conditions. At these concentrations chlorogenic acid significantly decreased platelet inflammatory mediators (sP-selectin, sCD40L, CCL5 and IL-1 beta) and increased intraplatelet cAMP levels/PKA activation. Interestingly, SQ22536 (an adenylate cyclase inhibitor) and ZM241385 (a potent A(2A) receptor antagonist) attenuated the antiplatelet effect of chlorogenic acid. Chlorogenic acid is compatible to the active site of the adenosine A(2A) receptor as revealed through molecular modeling. In addition, chlorogenic acid had a significantly lower effect on mouse bleeding time when compared to the same dose of aspirin. Conclusions: Antiplatelet and antithrombotic effects of chlorogenic acid are associated with the A(2A) receptor/adenylate cyclase/cAMP/PKA signaling pathway.

引用

页数：13

共 49 条

[41] In the search of new functional food ingredients from algae [J].

Plaza, Merichel ;

Cifuentes, Alejandro ;

Ibanez, Elena .

TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2008, 19 (01) :31-39

[42] Adaptation of a photochemical method to initiate recurrent platelet-mediated thrombosis in small animals [J].

Przyklenk, Karin ;

Whittaker, Peter .

LASERS IN MEDICAL SCIENCE, 2007, 22 (01) :42-45

[43] The aspirin controversy in primary prevention [J].

Raju, Nina C. ;

Eikelboom, John W. .

CURRENT OPINION IN CARDIOLOGY, 2012, 27 (05) :499-507

[44] Risk of bleeding complications with antiplatelet agents: Meta-analysis of 338,191 patients enrolled in 50 randomized controlled trials [J].

Serebruany, VL ;

Malinin, AI ;

Eisert, RM ;

Sane, DC .

AMERICAN JOURNAL OF HEMATOLOGY, 2004, 75 (01) :40-47

[45]

WALTER U, 1993, ADV EXP MED BIOL, V344, P237

[46] Distributed wide-area multi-object tracking with non-overlapping camera views [J].

Wang, Youlu ;

Velipasalar, Senem ;

Gursoy, Mustafa Cenk .

MULTIMEDIA TOOLS AND APPLICATIONS, 2014, 73 (01) :7-39

[47] The blood pressure-lowering effect and safety of chlorogenic acid from green coffee bean extract in essential hypertension [J].

Watanabe, Takuya ;

Arai, Yoichi ;

Mitsui, Yuki ;

Kusaura, Tatsuya ;

Okawa, Wataru ;

Kajihara, Yasushi ;

Saito, Ikuo .

CLINICAL AND EXPERIMENTAL HYPERTENSION, 2006, 28 (05) :439-449

[48]

World Health Organization, 2004, WHO PUBL DEF ATL GLO

[49] A new role for the A2b adenosine receptor in regulating platelet function [J].

Yang, D. ;

Chen, H. ;

Koupenova, M. ;

Carroll, S. H. ;

Eliades, A. ;

Freedman, J. E. ;

Toselli, P. ;

Ravid, K. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2010, 8 (04) :817-827

← 1 2 3 4 5 →