Radiosynthesis of 3′-deoxy-3′-[18F]fluorothymidine:: [18F]FLT for imaging of cellular proliferation in vivo

被引：167
|
作者：
Grierson, JR
Shields, AF
机构：
[1] Univ Washington, Med Ctr, Res Imaging Lab, Seattle, WA 98195 USA
[2] Wayne State Univ, Harper Hosp, Karamanos Canc Inst, Detroit, MI USA
来源：
NUCLEAR MEDICINE AND BIOLOGY | 2000年 / 27卷 / 02期
关键词：
F-18]FLT; 3 ' deoxy-3 '-flourothymidine; DNA synthesis; PET imaging; proliferation; fluorine-18; TK-1; thymidine kinase; thymidine metabolism; C-11]thymidine;
D O I：
10.1016/S0969-8051(99)00104-3
中图分类号：
R8 [特种医学]; R445 [影像诊断学];
学科分类号：
1002 ; 100207 ; 1009 ;
摘要：
A reliable radiosynthesis of 3'-deoxy-3'-[F-18]fluorothymidine ([F-18]FLT) has been developed based on [F-18]fluoride displacement of a protected nosylate precursor. A simple three-step synthesis is described that is useful for preparing >10 mCi (370 MBq) of radiochemically pure [F-18]FLT, with a specific activity >1 Ci/mu mol (37 GBq/mu mol) at EOS within 100 min and in 13% radiochemical yield tend of bombardment (EOB); 7% end of synthesis (EOS). [F-18]FLT has been designed as a new positron emission tomography imaging agent for visualizing cellular proliferation in vivo based on the metabolism of thymidine. NUCL MED BIOL 27;2:143-156, 2000. (C) 2000 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：143 / 156
页数：14
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