Identification of bioactive peptides from brewers' spent grain and contribution of Leu/Ile to bioactive potency

被引：52

作者：

Cermeno, Maria ^{[1
]}

Connolly, Alan ^{[1
]}

O'Keeffe, Martina B. ^{[1
]}

Flynn, Cal ^{[2
]}

Alashi, Adeola M. ^{[3
]}

Aluko, Rotimi E. ^{[3
]}

FitzGerald, Richard J. ^{[1
]}

机构：

[1] Univ Limerick, Sch Nat Sci, Dept Biol Sci, Limerick, Ireland

[2] Kerry Ingredients, Listowel, Kerry, Ireland

[3] Univ Manitoba, Dept Food & Human Nutr Sci, Winnipeg, MB R3T 2N2, Canada

来源：

JOURNAL OF FUNCTIONAL FOODS | 2019年 / 60卷

关键词：

Brewers spent grain; Hypotensive; Antioxidant; Peptide; ACE; DPP-IV; SHR; I-CONVERTING-ENZYME; ACE INHIBITORY PEPTIDES; NUTRITIONAL PROPERTIES; PROLYL ENDOPROTEINASE; PROTEIN HYDROLYSATE; VITRO; CARBOHYDRASES; GENERATION; SILICO; FOOD;

D O I：

10.1016/j.jff.2019.103455

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Brewer's spent grain (BSG) is a co-product of the brewing industry and is a potentially rich source of bioactive peptides. A BSG protein hydrolysate, manufactured at a semi-pilot scale, mediated significant hypotensive effects 6 h after ingestion in spontaneously hypertensive rats. This hydrolysate was fractionated and further analysed by UPLC-ESI-MS/MS for peptide identification. The peptides, IPY and LPY demonstrated the highest antioxidant activity (0.37 +/- 0.02 and 0.34 +/- 0.02 mu mol Trolox equivalents mu M peptide(-1), respectively), IPLQP and LPLQP had the highest ACE inhibitory activity (IC50: 3.10 +/- 0.60 and 3.17 +/- 0.60 mu M, respectively) while IPVP had the highest DPP-IV inhibitory activity (IC50: 38.67 +/- 5.94 mu M). Overall, peptides containing I had higher in vitro bioactivities when compared to peptides having L. Novel BSG peptides were identified with potential for application as naturally derived ingredients for the management of cardiovascular disease and type 2 diabetes.

引用

页数：9

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