Metabolic profile and antioxidant responses during drought stress recovery in sugarcane treated with humic acids and endophytic diazotrophic bacteria

被引：69
作者：
Aguiar, N. O. [1 ]
Medici, L. O. [2 ]
Olivares, F. L. [1 ]
Dobbss, L. B. [3 ]
Torres-Netto, A. [1 ]
Silva, S. F. [1 ]
Novotny, E. H. [4 ]
Canellas, L. P. [1 ]
机构：
[1] Univ Estadual Norte Fluminense Darcy Ribeiro UENF, Nucleo Desenvolvimento Insumos Biol Agr NUDIBA, Ave Alberto Lamego 2000, BR-28013602 Rio De Janeiro, Brazil
[2] Univ Fed Rural Rio de Janeiro, Dept Ciencias Fisiol, Rio De Janeiro, Brazil
[3] UVV, Lab Microbiol Ambiental & Biotecnol, Boa Vista, Brazil
[4] Embrapa Solos, Rua Jardim Bot, Rio De Janeiro, Brazil
来源：
ANNALS OF APPLIED BIOLOGY | 2016年 / 168卷 / 02期
关键词：
Humic substances; nitrogen-fixing bacteria; water stress alleviation; water use efficiency; GROWTH-PROMOTING BACTERIA; PLANT-GROWTH; OSMOTIC ADJUSTMENT; WATER-STRESS; SUBSTANCES; MECHANISMS; RESISTANCE; TOLERANCE; LEAVES; HERBASPIRILLUM;
D O I：
10.1111/aab.12256
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Water deficit is the major yield-limiting factor for sugarcane crop production that can be enhanced by inoculating with plant growth promoting bacteria (PGPB) combined with humic substances. The aim of this work was to examine changes to the metabolic profile and antioxidant enzyme activity of sugarcane treated with PGPB and humic acid (HA) after drought and then rehydration. The drought was imposed by withholding irrigation for 21 days thereby measuring enzyme activity, metabolic profile and photosynthetic rate 1 week after rehydratation. Growth of plants treated with HA, PGPB and with both treatments combined (PGPB+HA) was higher than control plants. The antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities remained higher after rehydration only in plants treated with HA. Plants treated with HA and PGPB+HA exhibited increased transpiration, stomatal conductance and net photosynthesis than plants treated with PGPB. The PGPB-treated plants exhibited drought resistance that resembled delayed stress onset', which is a new term for preserving water in the plants tissues. Water preservation in plants treated with PGPB was corroborated by higher relative water content (RWC) than control plants at the end of the drought period. Plants treated with HA+PGPB exhibited the highest water potential after rehydration and high RWC. Osmotic adjustment in the other treatments (control, HA and PGPB) was indicated by a new pattern of metabolic response after rehydration, including generally enhanced carbohydrates and proteins and specific changes induced by HA-enhancing aromatic compounds, whereas PGPB exhibited enhanced fatty acids and other aliphatic H species. Humic acids assist with drought stress recovery by inducing antioxidant enzyme activity whereas PGPB induced preservation of leaf water potential and RWC by closing stomata efficiently, resulting in plant water preservation.
引用
收藏
页码：203 / 213
页数：11
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