Wheat flour peroxidase is a prominent allergen associated with baker's asthma

被引:0
|
作者
SanchezMonge, R
GarciaCasado, G
LopezOtin, C
Armentia, A
Salcedo, G
机构
[1] UPM, ETS INGN AGRON, DEPT BIOTECNOL, UNIDAD BIOQUIM, MADRID 28040, SPAIN
[2] UNIV OVIEDO, FAC MED, DEPT BIOQUIM & BIOL MOL, OVIEDO, SPAIN
[3] HOSP RIO ORTEGA, SECC ALERGIA, VALLADOLID, SPAIN
来源
CLINICAL AND EXPERIMENTAL ALLERGY | 1997年 / 27卷 / 10期
关键词
allergen; baker's asthma; cereal; complex glycans; seed peroxidase; wheat;
D O I
暂无
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background Putative wheat allergens with molecular sizes around 35 kDa have been repeatedly detected in immunoblots using sera from patients with baker's asthma. However, none of these allergens had been previously isolated. Objective To purify and characterize a major component of the 35 kDa allergenic band which seems to be present in flour from diploid, tetraploid (pasta) and hexaploid (bread) wheats. Methods Gel-filtration chromatography followed by RP-HPLC have allowed the purification of a new wheat allergen. Immunodetection with sera from allergic patients and with a specific serum for asparagine-linked complex glycans, as well as amino acid sequencing, were used to identify the isolated protein. Results A 36 kDa allergen has been purified from wheat flour. Its N-terminal and internal sequences, N-linked glycans, and enzymatic activity indicated that this allergen is a seed-specific peroxidase. Sera from six out of 10 patients hypersensitive to wheat flour displayed positive reactions when assayed against the purified dot-blotted allergen. Conclusion Seed-specific peroxidases from cereals (flours) seem to be important allergens in hypersensitive diseases associated with the manipulation of these plant materials. A new enzyme has thus been involved in IgE-mediated diseases.
引用
收藏
页码:1130 / 1137
页数:8
相关论文
共 50 条
  • [1] Wheat flour immunotherapy in Baker's asthma
    Swaminathan, S.
    Heddle, R. J.
    INTERNAL MEDICINE JOURNAL, 2007, 37 (09) : 663 - 664
  • [2] Molecular Characterization of Major Allergens Associated with Baker's Asthma in Wheat Flour
    Wang, Ji-Rui
    Wei, Yu-Ming
    Fedak, George
    Liu, Zhi-Gang
    Jiang, Qian-Tao
    Pu, Zhi-En
    Zheng, You-Liang
    FOOD SCIENCE AND TECHNOLOGY RESEARCH, 2010, 16 (04) : 341 - 346
  • [3] Wheat lipid transfer protein is a major allergen associated with baker's asthma
    Palacin, Arantxa
    Quirce, Santiago
    Armentia, Alicia
    Fernandez-Nieto, Mar
    Pacios, Luis F.
    Asensio, Teresa
    Sastre, Joaquin
    Diaz-Perales, Araceli
    Salcedo, Gabriel
    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2007, 120 (05) : 1132 - 1138
  • [4] Baker's asthma - differences in allergen profiles to recombinant wheat flour proteins in German and Spanish bakers
    Sander, I
    Rozynek, P.
    Rihs, H.
    van Kampen, V
    Meurer, U.
    Quirce, S.
    Bruening, T.
    Raulf-Heimsoth, M.
    ALLERGY, 2010, 65 : 57 - 57
  • [5] A further wheat allergen for baker's asthma: Tri a 40
    Sander, Ingrid
    Rihs, Hans-Peter
    Bruening, Thomas
    Raulf, Monika
    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2016, 137 (04) : 1286 - 1286
  • [6] Wheat Allergens Associated With Baker's Asthma
    Salcedo, G.
    Quirce, S.
    Diaz-Perales, A.
    JOURNAL OF INVESTIGATIONAL ALLERGOLOGY AND CLINICAL IMMUNOLOGY, 2011, 21 (02) : 81 - 92
  • [7] Identification of wheat gliadins as an allergen family related to baker's asthma
    Bittner, Cordula
    Grassau, Britta
    Frenzel, Karsten
    Baur, Xaver
    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2008, 121 (03) : 744 - 749
  • [8] Specific inhalation challenge with wheat flour in workers with suspected baker's asthma
    R. De Zotti
    M. Bovenzi
    C. Negro
    A. Cirla
    A. Innocenti
    A. Lorusso
    A. Mariano
    P. L. Paggiaro
    D. Talini
    G. Pisati
    C. Romano
    F. Sulotto
    International Archives of Occupational and Environmental Health, 1999, 72 : 335 - 337
  • [9] Component-resolved diagnosis of wheat flour allergy in baker's asthma
    Gomez-Casado, Cristina
    Garrido-Arandia, Maria
    Pereira, Catia
    Catarino, Manuela
    Parro, Victor
    Armentia, Alicia
    Quirce, Santiago
    Armentia, Blanca
    Barranco, Paloma
    Blanca, Miguel
    Campo, Paloma
    Diaz-Perales, Araceli
    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2014, 134 (02) : 480 - 483
  • [10] Clinical evaluation of wheat flour specific IgE antibody in baker's asthma
    Kamidaira, T
    Suetsugu, S
    Kako, K
    Sakakibara, H
    ADVANCES IN THE PREVENTION OF OCCUPATIONAL RESPIRATORY DISEASES, 1998, 1153 : 787 - 789