CULTURE ENVIRONMENT, IBA CONCENTRATION AND CULTIVATION TIME ON IN VITRO ROOTING OF BLACKBERRY AND RASPBERRY

The propagation of blackberry and raspberry occur mainly through root cutting and even of softwoods stem; however. it is already increasing the interest for the use of the micropropagation as an alternative technique of vegetative propagation of fruit plants, since the rooting is one of the most difficult stages, in which the definition of both the culture environment and the best concentration of AIB for the rooting, is an important stage. For that purpose, the aim of this research was to determine the best cultivation type and the best concentration of IBA in the culture environment for the in vitro rooting of the blackberry 'Xavante' and the raspberry 'Batum' and 'Heritage'. The vegetable material used was atex microcutting with two leaves, measuring about I cm of length, originated from the in vitro cultivation. The studied factors were the kinds of culture (MS and WPM), the IBA concentration in the culture environment and the time of cultivation of the rnicrocutting in an environment with IBA. The WPM environment, in low concentrations, less than 3 mu M of IBA, together with T1, induced larger rooting averages and length. High concentrations of AIB induced the callus formation for blackberry 'Xavante'. For raspberry, using WPM environment with smaller concentrations of AIB (0 and 3 ,mu M), it was shown the best average in the number of roots, length of roots and small callus intensity, with the largest concentrations of IBA it happened larger callus emergence.