Novel SlVmac DNA vaccines encoding Env, Pol and Gag consensus proteins elicit strong cellular immune responses in cynomolgus macaques

被引：13
|
作者：
Yan, Jian [1 ]
Hokey, David A. [1 ]
Morrow, Matthew R. [1 ]
Corbitt, Natasha [1 ]
Harris, Kristina [1 ]
Harris, Drea [1 ]
Weiner, David B. [1 ]
机构：
[1] Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA
来源：
VACCINE | 2009年 / 27卷 / 25-26期
基金：
美国国家卫生研究院;
关键词：
SIV DNA vaccine; Consensus; Cellular immune responses; ENVELOPE GLYCOPROTEIN; SURFACE EXPRESSION; VIRAL REPLICATION; RHESUS MACAQUES; AIDS VACCINE; CODON USAGE; VIRUS; IMMUNOGENICITY; IMMUNIZATION; CHALLENGE;
D O I：
10.1016/j.vaccine.2009.01.065
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Cellular immunity plays an important role in controlling HIV-1 replication. One of the major challenges in developing an HIV-1 DNA vaccine is to generate broader and more potent cellular responses. In this study, we constructed three novel constructs expressing SIVmac antigens Env, Pol and Gag, respectively, with the goal of increasing anti-SIV cellular immunity. The results demonstrate that these constructs can induce strong cellular immune responses in a murine model. Moreover, when applied to cynomolgus macaques, these constructs are not only able to elicit robust IFN-gamma effector responses, but also induce SIV antigen-specific CD8(+) T cells that have high Proliferative capacity. These data suggest that such DNA immunogens deserve further examination for their potential to control viral replication. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：3260 / 3266
页数：7
相关论文
共 42 条
  • [31] Evaluation of humoral, mucosal, and cellular immune responses following co-immunization of HIV-1 Gag and Env proteins expressed by Newcastle disease virus (vol 11, pg 504, 2015)
    Khattar, Sunil K.
    Palaniyandi, Senthilkumar
    Samal, Sweety
    LaBranche, Celia C.
    Montefiori, David C.
    Zhu, Xiaoping
    Samal, Siba K.
    HUMAN VACCINES & IMMUNOTHERAPEUTICS, 2016, 12 (04) : 1087 - 1087
  • [32] DNA vaccines encoding APC-targeted ErbB2 and NY-ESO-1 fusion proteins induce anti tumor immune responses
    Sloots, A.
    Mastini, C.
    Rohrbach, F.
    Curcio, C.
    Weth, R.
    Jaeger, E.
    Forni, G.
    Cavallo, F.
    Wels, W. S.
    CANCER IMMUNOLOGY IMMUNOTHERAPY, 2007, 56 (03) : 411 - 411
  • [33] Cellular immunity induced by a novel HPV18 DNA vaccine encoding an E6/E7 fusion consensus protein in mice and rhesus macaques
    Yan, Jian
    Harris, Kristina
    Khan, Amir S.
    Draghia-Akli, Ruxandra
    Sewell, Duane
    Weiner, David B.
    VACCINE, 2008, 26 (40) : 5210 - 5215
  • [34] Immune profile and responses of a novel dengue DNA vaccine encoding an EDIII-NS1 consensus design based on Indo-African sequences
    Sankaradoss, Arun
    Jagtap, Suraj
    Nazir, Junaid
    Moula, Shefta E.
    Modak, Ayan
    Fialho, Joshuah
    Iyer, Meenakshi
    Shastri, Jayanthi S.
    Dias, Mary
    Gadepalli, Ravisekhar
    Aggarwal, Alisha
    Vedpathak, Manoj
    Agrawal, Sachee
    Pandit, Awadhesh
    Nisheetha, Amul
    Kumar, Anuj
    Bordoloi, Mahasweta
    Shafi, Mohamed
    Shelar, Bhagyashree
    Balachandra, Swathi S.
    Damodar, Tina
    Masika, Moses Muia
    Mwaura, Patrick
    Anzala, Omu
    Muthumani, Kar
    Sowdhamini, Ramanathan
    Medigeshi, Guruprasad R.
    Roy, Rahul
    Pattabiraman, Chitra
    Krishna, Sudhir
    Sreekumar, Easwaran
    MOLECULAR THERAPY, 2022, 30 (05) : 2058 - 2077
  • [35] Humoral and Cellular Immune Responses Induced by Bivalent DNA Vaccines Expressing Fusion Capsid Proteins of Porcine Circovirus Genotypes 2a and 2b
    Meas, Sochanwattey
    Chaimongkolnukul, Khuanjit
    Narkpuk, Jaraspim
    Mekvichitsaeng, Phenjun
    Poomputsa, Kanokwan
    Wanasen, Nanchaya
    Roshorm, Yaowaluck Maprang
    VACCINES, 2024, 12 (03)
  • [36] Immunization with plasmid DNA encoding a truncated, secreted form of the bovine viral diarrhea virus E2 protein elicits strong humoral and cellular immune responses
    Liang, R
    van den Hurk, JV
    Zheng, CF
    Yu, H
    Pontarollo, RA
    Babiuk, LA
    Littel-van den Hurk, SV
    VACCINE, 2005, 23 (45) : 5252 - 5262
  • [37] An SIV DNA vaccine co-administered with an IL-15 or IL-12 expression plasmid enhances CD8 SIV-specific cellular immune responses in cynomolgus macaques
    不详
    JOURNAL OF MEDICAL PRIMATOLOGY, 2005, 34 (5-6) : 325 - 325
  • [38] Strong HCV NS3/4a, NS4b, NS5a, NS5b-specific cellular immune responses induced in Rhesus macaques by a novel HCV genotype 1a/1b consensus DNA vaccine
    Latimer, Brian
    Toporovski, Roberta
    Yan, Jian
    Pankhong, Panyupa
    Morrow, Matthew P.
    Khan, Amir S.
    Sardesai, Niranjan Y.
    Welles, Seth L.
    Jacobson, Jeffrey M.
    Weiner, David B.
    Kutzler, Michele A.
    HUMAN VACCINES & IMMUNOTHERAPEUTICS, 2014, 10 (08) : 2357 - 2365
  • [39] Cynomolgus macaques immunized with two HIV-1 Tat stabilized proteins raise strong and long-lasting immune responses with a pattern of Th1/Th2 response differing from that in mice
    Turbant, Sabrina
    Martinon, Frederic
    Moine, Gervaise
    Le Grand, Roger
    Leonetti, Michel
    VACCINE, 2009, 27 (39) : 5349 - 5356
  • [40] Efficacy of a New Generation of DNA-Vaccines Encoding Retrovirus-Based Virus-Like Particles to Induce Both Cellular and Humoral Immune Responses and Its Use for Antiviral Immunotherapies
    Huret, Christophe
    Miyalou, Mathilde
    Torrieri, Lea
    Desjardins, Delphine
    Dalba, Charlotte
    Klatzmann, David
    Bellier, Bertrand
    HUMAN GENE THERAPY, 2010, 21 (06) : 783 - 784
12345