Novel SlVmac DNA vaccines encoding Env, Pol and Gag consensus proteins elicit strong cellular immune responses in cynomolgus macaques

被引：13
|
作者：
Yan, Jian [1 ]
Hokey, David A. [1 ]
Morrow, Matthew R. [1 ]
Corbitt, Natasha [1 ]
Harris, Kristina [1 ]
Harris, Drea [1 ]
Weiner, David B. [1 ]
机构：
[1] Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA
来源：
VACCINE | 2009年 / 27卷 / 25-26期
基金：
美国国家卫生研究院;
关键词：
SIV DNA vaccine; Consensus; Cellular immune responses; ENVELOPE GLYCOPROTEIN; SURFACE EXPRESSION; VIRAL REPLICATION; RHESUS MACAQUES; AIDS VACCINE; CODON USAGE; VIRUS; IMMUNOGENICITY; IMMUNIZATION; CHALLENGE;
D O I：
10.1016/j.vaccine.2009.01.065
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Cellular immunity plays an important role in controlling HIV-1 replication. One of the major challenges in developing an HIV-1 DNA vaccine is to generate broader and more potent cellular responses. In this study, we constructed three novel constructs expressing SIVmac antigens Env, Pol and Gag, respectively, with the goal of increasing anti-SIV cellular immunity. The results demonstrate that these constructs can induce strong cellular immune responses in a murine model. Moreover, when applied to cynomolgus macaques, these constructs are not only able to elicit robust IFN-gamma effector responses, but also induce SIV antigen-specific CD8(+) T cells that have high Proliferative capacity. These data suggest that such DNA immunogens deserve further examination for their potential to control viral replication. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：3260 / 3266
页数：7
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