Sample displacement chromatography of Atlantic Salmon (Salmo salar) thrombin

被引：13
|
作者：
Manseth, E
Skjervold, PO
Flengsrud, R
机构：
[1] Agr Univ Norway, Dept Chem Biotechnol & Food Sci, N-1432 As, Norway
[2] Agr Univ Norway, Dept Math Sci & Technol, N-1432 As, Norway
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2004年 / 60卷 / 01期
关键词：
protein purification; salmon; sample displacement chromatography; thrombin;
D O I：
10.1016/j.jbbm.2004.04.016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A modified method of sample displacement chromatography (SDC) was used to purify active salmon thrombin on a heparin-coupled matrix to near homogeneity in milligram amounts from 117 ml plasma. This was achieved by combining a low-pressure multi-column affinity chromatography system with non-homogenous sample application in the order of increasing affinity to Heparin Sepharose. The results suggest that this modified method could be useful in protein purification. Some characteristics of salmon thrombin are presented. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：39 / 47
页数：9
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