Polymorphic microsatellite loci in the coffee berry borer, Hypothenemus hampei (Coleoptera, scolytidae)

被引：7
|
作者：
Gauthier, N [1 ]
Rasplus, JY [1 ]
机构：
[1] CBGP, F-34988 Montferrier Sur Lez, France
来源：
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 02期
关键词：
genetic diversity; Hypothenemus; inbreeding; microsatellite; Scolytidae;
D O I：
10.1111/j.1471-8286.2004.00647.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Microsatellite loci were isolated from the coffee berry borer, Hypothenemus hampei, which is regarded as the major pest in coffee cultures. Seven polymorphic loci were obtained from an enriched genomic library. A low to moderate genetic diversity was observed per locus, with an observed number of alleles ranging from two to five in the 39 unrelated individuals sampled from Ethiopia. A clear deficit of heterozygotes within the population (mean heterozygosities, H-O = 0.10/H-E = 0.50) and an extreme inbreeding (F-IS, 0.70-1.00) were demonstrated. Cross-species amplifications showed that some of the markers could be useful in two closely related Hypothenemus species.
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页码：294 / 296
页数：3
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