Fructose-arginine, a non-saponin molecule of Korean Red Ginseng, attenuates AIM2 inflammasome activation

被引:19
作者
Ahn, Huijeong [1 ,2 ]
Han, Byung-Cheol [1 ,2 ,3 ]
Lee, Seung-Ho [3 ]
Lee, Geun-Shik [1 ,2 ]
机构
[1] Kangwon Natl Univ, Coll Vet Med, Chunchon, South Korea
[2] Kangwon Natl Univ, Inst Vet Sci, Chunchon, South Korea
[3] Korea Ginseng Corp, Korea Ginseng Res Inst, Daejeon, South Korea
基金
新加坡国家研究基金会;
关键词
Korean Red Ginseng extract; Non-saponin; Fructose-arginine; AIM2; inflammasome; NLRP3; DOMAIN; INFECTION; RESPONSES; SENSOR; NLRC4;
D O I
10.1016/j.jgr.2020.06.002
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background: Korean Red Ginseng extract (RGE) has been reported to act as an inflammasome modulator. Ginsenosides, saponin molecules of RGE, selectively inhibit activation of NLRP3 and AIM2 inflammasomes, while non-saponin molecules of RGE upregulate inflammasome components associated with the initiation of NLRP3 inflammasome activation. In this study, we investigated the effect of non-saponin components of RGE on AIM2 inflammasome activation. Methods: The role of non-saponins of RGE on AIM2 inflammasomes was tested in mouse bone marrow-derived macrophages, a human monocyte-like cell line, and a mouse animal model. Cells or mice were transfected with dsDNA or inoculated with Listeria monocytogenes to activate AIM2 inflammasomes. Several indices of inflammasome activation were examined via immunoblot or ELISA analysis. Results: The non-saponin fraction and saponin-eliminating fraction (SEF) of RGE selectively attenuated the activation of AIM2 inflammasomes, but not that of NLRP3 or NLRC4 inflammasomes. Fructose-arginine, an amino-sugar, was shown to be effective against AIM2 inflammasome activation. Conclusion: Non-saponins of RGE, such as fructose-arginine, might be effective in regulating infectious and autoimmune diseases resulting from AIM2 inflammasome activation. (C) 2020 The Korean Society of Ginseng. Publishing services by Elsevier B.V.
引用
收藏
页码:808 / 814
页数:7
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