Multimodal evaluation of ultra-short laser pulses treatment for skin burn injuries

被引：18

作者：

dos Santos, Moises Oliveira ^{[1
,2
]}

Latrive, Anne ^{[2
]}

Augusto de Castro, Pedro Arthur ^{[2
]}

de Rossi, Wagner ^{[2
]}

Tenorio Zorn, Telma Maria ^{[3
]}

Samad, Ricardo Elgul ^{[2
]}

Freitas, Anderson Zanardi ^{[2
]}

Cesar, Carlos Lenz ^{[4
,5
]}

Vieira Junior, Nilson Dias ^{[2
]}

Zezell, Denise Maria ^{[2
]}

机构：

[1] Univ Estado Amazonas, Escola Super Tecnol, Manaus, Amazonas, Brazil

[2] Ctr Lasers & Aplicacoes, Inst Pesquisas Energet & Nucl, Sao Paulo, SP, Brazil

[3] Univ Sao Paulo, Inst Ciencias Biomed, Sao Paulo, SP, Brazil

[4] Univ Estadual Campinas, Inst Fis Gleb Wataghin, Campinas, SP, Brazil

[5] Univ Fed Ceara, Dept Fis, Fortaleza, Ceara, Brazil

来源：

BIOMEDICAL OPTICS EXPRESS | 2017年 / 8卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

OPTICAL COHERENCE TOMOGRAPHY; HUMAN ADIPOSE-TISSUE; COLLAGEN FIBER; GENERATION; DIFFERENTIATION; ATTENUATION; MICROSCOPY; CELLS; SPECTROSCOPY; MECHANISMS;

D O I：

10.1364/BOE.8.001575

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Thousands of people die every year from burn injuries. The aim of this study is to evaluate the feasibility of high intensity femtosecond lasers as an auxiliary treatment of skin burns. We used an in vivo animal model and monitored the healing process using 4 different imaging modalities: histology, Optical Coherence Tomography (OCT), Second Harmonic Generation (SHG), and Fourier Transform Infrared (FTIR) spectroscopy. 3 dorsal areas of 20 anesthetized Wistar rats were burned by water vapor exposure and subsequently treated either by classical surgical debridement, by laser ablation, or left without treatment. Skin burn tissues were non-invasively characterized by OCT images and biopsied for further histopathology analysis, SHG imaging and FTIR spectroscopy at 3, 5, 7 and 14 days after burn. The laser protocol was found as efficient as the classical treatment for promoting the healing process. The study concludes to the validation of femtosecond ultra-short pulses laser treatment for skinburns, with the advantage of minimizing operatory trauma. (C) 2017 Optical Society of America

引用

页码：1575 / 1588

页数：14

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