Altered rainfall patterns, gas exchange, and growth in grasses and forbs

Although the potential for increased temperature is the primary and best-studied aspect of anthropogenic climate change, altered rainfall patterns, increased storm intensity, and more severe droughts are also predicted in most climate-change scenarios. We altered experimentally the rainfall regime in a native tallgrass prairie in northeastern Kansas and assessed leaf-level physiological activity and plant growth responses for C-3 and C-4 plant species. Our primary objective was to contrast the importance of reductions in rainfall quantity (30% smaller rain events, no change in rainfall pattern) with an altered, more extreme distribution of rainfall (no reduction in total growing-season quantity, 50% increased inter-rainfall dry intervals) for these dominant species from the two main plant functional groups (C-4 grasses, C-3 forbs) present in many grasslands. Leaf water potential (psi(1)), net photosynthetic carbon gain (A(CO2)), specific leaf mass, leaf C : N ratio, growth rate for Andropogon gerardii (C-4 grass) and Solidago canadensis (C-3 forb), vegetative and flowering stem densities, and canopy light penetration for grass and forb assemblages were intensively monitored during the 1999 growing season in a long-term rainfall manipulation study at the Konza Prairie Biological Station. Soil water content at 0-30 cm depth was more variable in response to the altered rainfall distribution compared to the reduced-quantity treatment. In S. canadensis, A(CO2), g(s) (stomatal conductance), A : Ci (leaf stomatal [CO2]), and A : E (estimated leaf transpiration rate) were positively correlated with soil water content, but no relationship was seen for A. gerardii, indicating that even though this dominant grass species has most of its roots in the upper 30 cm of the soil, A. gerardii was buffered physiologically from increased resource variability. There were few significant responses in growth parameters in either grasses or forbs, but canopy light penetration increased with both rainfall treatments. We concluded (1) that the temporal variability in rainfall inputs can have as much impact on soil moisture as simple reductions in rainfall quantities with no change in temporal distribution, (2) that responses of A. gerardii and S. canadensis to altered rainfall distributions were not consistent with common views of soil resource partitioning between shallow-rooted grasses and deep-rooted forbs, and (3) that altered rainfall patterns may have the potential to offset elevated CO2 impacts on grassland vegetation.