Purification and characterization of lignin peroxidase from Loweporus lividus MTCC-1178

被引：18
|
作者：
Yadav, Meera [1 ]
Yadav, Pratibha [2 ]
Yadav, Kapil Deo Singh [1 ]
机构：
[1] DDU Gorakhpur Univ, Dept Chem, Gorakhpur 273009, Uttar Pradesh, India
[2] Udai Pratap Coll, Dept Chem, Varanasi 221002, Uttar Pradesh, India
来源：
ENGINEERING IN LIFE SCIENCES | 2009年 / 9卷 / 02期
关键词：
Lignin peroxidase; Loweporus lividus; Metalloenzyme; Peroxidase; WHITE-ROT FUNGI; ENZYMES;
D O I：
10.1002/elsc.200800084
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Lignin peroxidase from the culture filtrate of Loweporus lividus MTCC-1178 has been purified to homogeneity using Amicon concentration and DEAE cellulose chromatography The molecular weight of the purified lignin peroxidase using SDS-PAGE analysis has been found to be 40 kDa. The K-m values for veratryl alcohol and H2O2 for the purified enzyme were 58 and 83 mu M, respectively. The calculated k(cat) value of the purified enzyme using veratryl alcohol as the substrate was 2.5 s(-1). The pH and temperature optima of lignin peroxidase have been found to be 2.6 and 24 degrees C, respectively.
引用
收藏
页码：124 / 129
页数：6
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