Direct electrochemical regeneration of the cofactor NADH on bare Ti, Ni, Co and Cd electrodes: The influence of electrode potential and electrode material

被引：84

作者：

Ali, Irshad ^{[1
]}

Khan, Tariq ^{[1
]}

Omanovic, Sasha ^{[1
]}

机构：

[1] McGill Univ, Dept Chem Engn, Montreal, PQ H3A 0C5, Canada

来源：

JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL | 2014年 / 387卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

Electrochemical 1,4-NADH regeneration; Non-modified metal electrodes; Electrode potential; Metal-hydrogen bond strength; Hydrogen surface coverage; NICOTINAMIDE-ADENINE-DINUCLEOTIDE; GLASSY-CARBON ELECTRODE; GOLD ELECTRODE; ELECTROENZYMATIC REGENERATION; LACTATE-DEHYDROGENASE; HYDROGEN EVOLUTION; REDOX MEDIATOR; COENZYME NADH; REDUCTION; NAD(+);

D O I：

10.1016/j.molcata.2014.02.029

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The regeneration of enzymatically-active reduced form of enzymatic cofactor nicotinamide adenine dinucleotide (1,4-NADH) from the oxidized form (NAD(+)) in a batch electrochemical reactor employing bare (non-modified) metal electrodes was investigated as a function of electrode potential and electrode material (Ti, Ni, Co and Cd). It was found that the regeneration of 1,4-NADH employing the electrodes is feasible; all the electrodes were capable of producing more than an 80% enzymatically-active product (1,4-NADH), reaching a 96% product purity on Ti. The product purity was found to be highly potential-, and material dependant. The origin of the material/potential dependency was related to the strength of the metal-hydrogen (M-H-ads) bond, and thus to the potential dependence of the Had, electrode surface coverage. In contradiction to literature, bare (non-modified) metal electrodes were found to be good candidates for electrochemical regeneration of enzymatically-active 1,4-NADH, when the regeneration is performed at a specific overpotential. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：86 / 91

页数：6

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