Efficient chemoenzymatic synthesis of globotriose and its derivatives with a recombinant α-(1→4)-galactosyltransferase

被引：35
|
作者：
Zhang, JB [1 ]
Kowal, P [1 ]
Fang, JW [1 ]
Andreana, P [1 ]
Wang, PG [1 ]
机构：
[1] Wayne State Univ, Dept Chem, Detroit, MI 48202 USA
来源：
CARBOHYDRATE RESEARCH | 2002年 / 337卷 / 11期
关键词：
globotriose; chemoenzymatic synthesis; LgtC; alpha-(1 -> 4)-galactosyltransferase; recombinant enzyme;
D O I：
10.1016/S0008-6215(02)00106-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A truncated alpha-(1-->4)-galactosyltransferase (LgtC) gene from Neisseria meningitidis was cloned. The recombinant glycosyltransferase was expressed in Escherichia coli BL21 (DE3) strain with high specific activity (5 units/mg protein). Its acceptor specificity was carefully characterized. Then the purified enzyme was utilized in highly efficient syntheses of globotriose and a variety of alpha-(1-->4)-galactosylated derivatives as potential antibacterial agents. (C) 2002 Published by Elsevier Science Ltd.
引用
收藏
页码：969 / 976
页数：8
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