StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity

被引：44

作者：

Samodelov, Sophia L. ^{[1
,2
]}

Beyer, Hannes M. ^{[2
,3
]}

Guo, Xiujie ^{[4
]}

Augustin, Maximilian ^{[3
,8
]}

Jia, Kun-Peng ^{[4
]}

Baz, Lina ^{[4
]}

Ebenhoeh, Oliver ^{[5
,6
]}

Beyer, Peter ^{[3
]}

Weber, Wilfried ^{[2
,3
,7
]}

Al-Babili, Salim ^{[4
]}

Zurbriggen, Matias D. ^{[1
]}

机构：

[1] Univ Dusseldorf, Inst Synthet Biol & Cluster Excellence Plant Sci, Dusseldorf, Germany

[2] Univ Freiburg, Spemann Grad Sch Biol & Med SGBM, Freiburg, Germany

[3] Univ Freiburg, Fac Biol, Freiburg, Germany

[4] KAUST, Biol & Environm Sci & Engn Div, Ctr Desert Agr, Thuwal 239556900, Saudi Arabia

[5] Univ Dusseldorf, Inst Quantitat & Theoret Biol, Dusseldorf, Germany

[6] Univ Dusseldorf, CEPLAS, Dusseldorf, Germany

[7] Univ Freiburg, BIOSS Ctr Biol Signalling Studies, Freiburg, Germany

[8] Roche Diagnost GmbH, Sandhofer Str 116, Mannheim, Germany

来源：

SCIENCE ADVANCES | 2016年 / 2卷 / 11期

关键词：

PLANT DEVELOPMENT; ARABIDOPSIS; BIOSYNTHESIS; PERCEPTION; HORMONE; CARLACTONE; RESPONSES; RICE; STEREOSPECIFICITY; DEGRADATION;

D O I：

10.1126/sciadv.1601266

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Strigolactones are key regulators of plant development and interaction with symbiotic fungi; however, quantitative tools for strigolactone signaling analysis are lacking. We introduce a genetically encoded hormone biosensor used to analyze strigolactone-mediated processes, including the study of the components involved in the hormone perception/signaling complex and the structural specificity and sensitivity of natural and synthetic strigolactones in Arabidopsis, providing quantitative insights into the stereoselectivity of strigolactone perception. Given the high specificity, sensitivity, dynamic range of activity, modular construction, ease of implementation, and wide applicability, the biosensor StrigoQuant will be useful in unraveling multiple levels of strigolactone metabolic and signaling networks.

引用

页数：8

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