Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells

被引：670

作者：

Simonsen, JL

Rosada, C

Serakinci, N

Justesen, J

Stenderup, K

Rattan, SIS

Jensen, TG

Kassem, M ^{[1
]}

机构：

[1] Aarhus Univ Hosp, Dept Endocrinol & Metab, DK-8000 Aarhus C, Denmark

[2] Aarhus Univ Hosp, Canc Cytogenet Lab, DK-8000 Aarhus, Denmark

[3] Univ Aarhus, Dept Mol & Struct Biol, Danish Ctr Mol Gerontol, DK-8000 Aarhus C, Denmark

[4] Univ Aarhus, Dept Human Genet, DK-8000 Aarhus C, Denmark

[5] Odense Univ Hosp, Dept Endocrinol & Metab, DK-5000 Odense C, Denmark

来源：

NATURE BIOTECHNOLOGY | 2002年 / 20卷 / 06期

关键词：

D O I：

10.1038/nbt0602-592

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human bone marrow stromal cells (hMSCs) were stably transduced by a retroviral vector containing the gene for the catalytic subunit of human telomerase (hTERT). Transduced cells (hMSC-TERTs) had telomerase activity, and the mean telomere length was increased as compared with that of control cells. The transduced cells have now undergone more than 260 population doublings (PD) and continue to proliferate, whereas control cells underwent senescence-associated proliferation arrest after 26 PD. The cells maintained production of osteoblastic markers and differentiation potential during continuous subculturing, did not form tumors, and had a normal karyotype. When implanted subcutaneously in immunodeficient mice, the transduced cells formed more bone than did normal cells. These results suggest that ectopic expression of telomerase in hMSCs prevents senescence-associated impairment of osteoblast functions.

引用

页码：592 / 596

页数：5

共 35 条

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