An Environmental DNA-Derived Type II Polyketide Biosynthetic Pathway Encodes the Biosynthesis of the Pentacyclic Polyketide Erdacin

被引：33
作者：
King, Ryan W. [1 ]
Bauer, John D. [1 ]
Brady, Sean F. [1 ]
机构：
[1] Rockefeller Univ, Lab Genetically Encoded Small Mol, New York, NY 10065 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 34期
关键词：
biosynthesis; DNA; metagenomics; natural products; polyketides; GENE CLUSTERS; NATURAL-PRODUCTS; SYNTHASE GENES; STREPTOMYCES-COELICOLOR; HETEROLOGOUS EXPRESSION; PROKARYOTIC DIVERSITY; SOIL; LIBRARIES; MICROORGANISMS; BACTERIA;
D O I：
10.1002/anie.200901209
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A down to earth approach: Environmental (from desert soil) DNA derived cosmid clones containing type II polyketide synthase genes were screened for their ability to produce clone-specific metabolites in Streptomyces. One of the Streptomyces recombinants produces erdacin (1), a polyketide with a previously unknown pentacyclic structure. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：6257 / 6261
页数：5
相关论文
共 29 条
  • [21] Metagenomic analysis reveals diverse polyketide synthase gene clusters in microorganisms associated with the marine sponge Discodermia dissoluta
    Schirmer, A
    Gadkari, R
    Reeves, CD
    Ibrahim, F
    DeLong, EF
    Hutchinson, CR
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (08) : 4840 - 4849
  • [22] A study of iterative type II polyketide synthases, using bacterial genes cloned from soil DNA: a means to access and use genes from uncultured microorganisms
    Seow, KT
    Meurer, G
    Gerlitz, M
    WendtPienkowski, E
    Hutchinson, CR
    Davies, J
    [J]. JOURNAL OF BACTERIOLOGY, 1997, 179 (23) : 7360 - 7368
  • [23] Shen B, 2000, TOP CURR CHEM, V209, P1
  • [24] COMPARISON OF PHENOTYPIC DIVERSITY AND DNA HETEROGENEITY IN A POPULATION OF SOIL BACTERIA
    TORSVIK, V
    SALTE, K
    SORHEIM, R
    GOKSOYR, J
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (03) : 776 - 781
  • [25] Prokaryotic diversity -: Magnitude, dynamics, and controlling factors
    Torsvik, V
    Ovreås, L
    Thingstad, TF
    [J]. SCIENCE, 2002, 296 (5570) : 1064 - 1066
  • [26] Identification of unique type II polyketide synthase genes in soil
    Wawrik, B
    Kerkhof, L
    Zylstra, GJ
    Kukor, JJ
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (05) : 2232 - 2238
  • [27] Biogeography of actinomycete communities and type II polyketide synthase genes in soils collected in New Jersey and Central Asia
    Wawrik, Boris
    Kudiev, Djumaniyaz
    Abdivasievna, Urinova A.
    Kukor, Jerome J.
    Zystra, Gerben J.
    Kerkhof, Lee
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (09) : 2982 - 2989
  • [28] Wilkinson SP, 2006, CURR ISSUES MOL BIOL, V8, P51
  • [29] (R)-PRECHRYSOPHANOL FROM ALOE-GRAMINICOLA
    YENESEW, A
    OGUR, JA
    DUDDECK, H
    [J]. PHYTOCHEMISTRY, 1993, 34 (05) : 1442 - 1444
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