Analysis of vitamin K in green tea leafs and infusions by SPME-GC-FID

被引:17
作者
Reto, Marcia
Figueira, Maria E.
Filipe, Helder M.
Almeida, Cristina M. M.
机构
[1] Univ Lisbon, Fac Farm, Unidade Farmacol & Farmacotoxicol, P-1600083 Lisbon, Portugal
[2] Univ Lisbon, Fac Farm, Lab Bromatol, P-1600083 Lisbon, Portugal
[3] Univ Lisbon, Fac Farm, Lab Hidrol, P-1600083 Lisbon, Portugal
关键词
green tea; SPME; infusion; vitamin K1; vitamin K2;
D O I
10.1016/j.foodchem.2005.09.016
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The usual methods for determination vitamin K are laborious. With the aim of replace them by a simpler method that requires fewer solvents, lower analytical levels and allows reporting of results in a smaller period of time, a procedure based on solid-phase microextraction (SPME) and gas chromatography with flame ionization detector (GC-FID) was developed. This method was used to analyse vitamin K in green tea leafs and infusions from nine brands of green tea commercialised in Portugal. The best analytical conditions were obtained using PDMS 7 mu m fibre using immersion extraction at 40 degrees C, for 45 min and 1300 rpm. The linear range for vitamins K1 and K2 was defined. The detection limits obtained with PDMS fibre and GC/FID were 0.16 and 0.07 mg/L for vitamins K1 and K2, respectively. In all analysed infusions the concentration of vitamin K was lower than the detection limit of the developed method, however, the concentration of vitamin K1 present in tea leafs was between 120 and 625 mu g/100 g. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:405 / 411
页数:7
相关论文
共 29 条
[1]  
[Anonymous], 1990, 84661 ISO
[2]  
Bolton-Smith C, 2000, BRIT J NUTR, V83, P389
[3]   TEA AND COFFEE BREWS ARE NOT DIETARY SOURCES OF VITAMIN-K-1 (PHYLLOQUINONE) [J].
BOOTH, S ;
MADABUSHI, HT ;
DAVIDSON, KW ;
SADOWSKI, JA .
JOURNAL OF THE AMERICAN DIETETIC ASSOCIATION, 1995, 95 (01) :82-83
[4]  
Booth Sarah L., 1993, Journal of Food Composition and Analysis, V6, P109, DOI 10.1006/jfca.1993.1014
[5]  
Booth SL, 1999, NUTR REV, V57, P288
[6]   Dietary intake and adequacy of vitamin K [J].
Booth, SL ;
Suttie, JW .
JOURNAL OF NUTRITION, 1998, 128 (05) :785-788
[7]   PHYLLOQUINONE (VITAMIN K-1) CONTENT OF FOODS IN THE US-FOOD-AND-DRUG-ADMINISTRATION TOTAL DIET STUDY [J].
BOOTH, SL ;
SADOWSKI, JA ;
PENNINGTON, JAT .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1995, 43 (06) :1574-1579
[8]  
Burtis C.A., 1994, TIETZ HDB CLIN CHEM
[9]   Determination of tea components with antioxidant activity [J].
Cabrera, C ;
Giménez, R ;
López, MC .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (15) :4427-4435
[10]   Tea and cancer prevention: Studies in animals and humans [J].
Chung, FL ;
Schwartz, J ;
Herzog, CR ;
Yang, YM .
JOURNAL OF NUTRITION, 2003, 133 (10) :3268S-3274S