Atomic force microscopy of swelling and hardening of intact erythrocytes fixed on substrate

被引:1
作者
Khalisov, M. M. [1 ,2 ]
Timoshchuk, K. I. [2 ]
Ankudinov, A. V. [2 ,3 ]
Timoshenko, T. E. [1 ]
机构
[1] Russian Acad Sci, Pavlov Inst Physiol, Nab Makarova 6, St Petersburg 199034, Russia
[2] ITMO Univ, Kronverkskii Pr 49, St Petersburg 197101, Russia
[3] Russian Acad Sci, Ioffe Physicotech Inst, Politekhnicheskaya Ul 26, St Petersburg 194021, Russia
基金
俄罗斯科学基金会;
关键词
STIFFNESS; CELLS;
D O I
10.1134/S1063784217020116
中图分类号
O59 [应用物理学];
学科分类号
摘要
Peak force measurements with the aid of atomic force microscopy are used to quantitatively map nanomechanical properties of intact erythrocytes of rats under conditions that are close to physiological conditions. Erythrocytes that are immobilized on the substrate preliminary processed using poly-L-lysine predominantly exhibit plane shape. However, cells may also exhibit stepwise transformation to semispherical objects with an increase in volume and hardening. Possible reasons for such transformations are discussed.
引用
收藏
页码:310 / 313
页数:4
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