Structural Role of Plasma Membrane Sterols in Osmotic Stress Tolerance of Yeast Saccharomyces cerevisiae

被引:9
作者
Sokolov, Svyatoslav S. S. [1 ]
Popova, Marina M. M. [2 ]
Pohl, Peter [3 ]
Horner, Andreas [3 ]
Akimov, Sergey A. A. [2 ]
Kireeva, Natalia A. A. [1 ]
Knorre, Dmitry A. A. [1 ]
Batishchev, Oleg V. V. [2 ]
Severin, Fedor F. F. [1 ]
机构
[1] Lomonosov Moscow State Univ, Belozersky Inst Physicochem Biol, 1-40 Leninskie Gory, Moscow 119991, Russia
[2] Russian Acad Sci, Frumkin Inst Phys Chem & Electrochem, 31-4 Leninskiyprospekt, Moscow 119071, Russia
[3] Johannes Kepler Univ Linz, Inst Biophys, Gruberstr 40, A-4020 Linz, Austria
基金
奥地利科学基金会; 俄罗斯科学基金会;
关键词
sterol; hyperosmotic stress; hypoosmotic stress; yeast; giant unilamellar vesicle; large unilamellar vesicle; light scattering; PERMEABILITY; CHOLESTEROL; ELASTICITY; LIPOSOMES; VESICLES; SIZE;
D O I
10.3390/membranes12121278
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Yeast S. cerevisiae has been shown to suppress a sterol biosynthesis as a response to hyperosmotic stress. In the case of sodium stress, the failure to suppress biosynthesis leads to an increase in cytosolic sodium. The major yeast sterol, ergosterol, is known to regulate functioning of plasma membrane proteins. Therefore, it has been suggested that the suppression of its biosynthesis is needed to adjust the activity of the plasma membrane sodium pumps and channels. However, as the sterol concentration is in the range of thirty to forty percent of total plasma membrane lipids, it is believed that its primary biological role is not regulatory but structural. Here we studied how lowering the sterol content affects the response of a lipid bilayer to an osmotic stress. In accordance with previous observations, we found that a decrease of the sterol fraction increases a water permeability of the liposomal membranes. Yet, we also found that sterol-free giant unilamellar vesicles reduced their volume during transient application of the hyperosmotic stress to a greater extent than the sterol-rich ones. Furthermore, our data suggest that lowering the sterol content in yeast cells allows the shrinkage to prevent the osmotic pressure-induced plasma membrane rupture. We also found that mutant yeast cells with the elevated level of sterol accumulated propidium iodide when exposed to mild hyperosmotic conditions followed by hypoosmotic stress. It is likely that the decrease in a plasma membrane sterol content stimulates a drop in cell volume under hyperosmotic stress, which is beneficial in the case of a subsequent hypo-osmotic one.
引用
收藏
页数:13
相关论文
共 37 条
  • [1] Pore formation in lipid membrane I: Continuous reversible trajectory from intact bilayer through hydrophobic defect to transversal pore
    Akimov, Sergey A.
    Volynsky, Pavel E.
    Galimzyanov, Timur R.
    Kuzmin, Peter I.
    Pavlov, Konstantin V.
    Batishchev, Oleg V.
    [J]. SCIENTIFIC REPORTS, 2017, 7
  • [2] Filter extrusion of liposomes using different devices: comparison of liposome size, encapsulation efficiency, and process characteristics
    Berger, N
    Sachse, A
    Bender, J
    Schubert, R
    Brandl, M
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2001, 223 (1-2) : 55 - 68
  • [3] Propidium uptake and ATP release in A549 cells share similar transport mechanisms
    Boudreault, Francis
    Tan, Ju Jing
    Grygorczyk, Ryszard
    [J]. BIOPHYSICAL JOURNAL, 2022, 121 (09) : 1593 - 1609
  • [4] Hog1: 20 years of discovery and impact
    Brewster, Jay L.
    Gustin, Michael C.
    [J]. SCIENCE SIGNALING, 2014, 7 (343)
  • [5] Osmotic Responses of Dunaliella to the Changes of Salinity
    Chen, Hui
    Jiang, Jian-Guo
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2009, 219 (02) : 251 - 258
  • [6] Osmotic shrinkage and reswelling of giant vesicles composed of dioleoylphosphatidylglycerol and cholesterol
    Claessens, M. M. A. E.
    Leermakers, F. A. M.
    Hoekstra, F. A.
    Stuart, M. A. Cohen
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2008, 1778 (04): : 890 - 895
  • [7] The HOG pathway and the regulation of osmoadaptive responses in yeast
    de Nadal, Eulalia
    Posas, Francesc
    [J]. FEMS YEAST RESEARCH, 2022, 22 (01)
  • [8] Nature of sterols affects plasma membrane behavior and yeast survival during dehydration
    Dupont, Sebastien
    Beney, Laurent
    Ferreira, Thierry
    Gervais, Patrick
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2011, 1808 (06): : 1520 - 1528
  • [9] ENTROPY-DRIVEN TENSION AND BENDING ELASTICITY IN CONDENSED-FLUID MEMBRANES
    EVANS, E
    RAWICZ, W
    [J]. PHYSICAL REVIEW LETTERS, 1990, 64 (17) : 2094 - 2097
  • [10] Functional profiling of the Saccharomyces cerevisiae genome
    Giaever, G
    Chu, AM
    Ni, L
    Connelly, C
    Riles, L
    Véronneau, S
    Dow, S
    Lucau-Danila, A
    Anderson, K
    André, B
    Arkin, AP
    Astromoff, A
    El Bakkoury, M
    Bangham, R
    Benito, R
    Brachat, S
    Campanaro, S
    Curtiss, M
    Davis, K
    Deutschbauer, A
    Entian, KD
    Flaherty, P
    Foury, F
    Garfinkel, DJ
    Gerstein, M
    Gotte, D
    Güldener, U
    Hegemann, JH
    Hempel, S
    Herman, Z
    Jaramillo, DF
    Kelly, DE
    Kelly, SL
    Kötter, P
    LaBonte, D
    Lamb, DC
    Lan, N
    Liang, H
    Liao, H
    Liu, L
    Luo, CY
    Lussier, M
    Mao, R
    Menard, P
    Ooi, SL
    Revuelta, JL
    Roberts, CJ
    Rose, M
    Ross-Macdonald, P
    Scherens, B
    [J]. NATURE, 2002, 418 (6896) : 387 - 391