Inositol Polyphosphate-4-Phosphatase Type I Negatively Regulates Phagocytosis via Dephosphorylation of Phagosomal PtdIns (3,4)P2

被引：12

作者：

Nigorikawa, Kiyomi ^{[1
]}

Hazeki, Kaoru ^{[1
]}

Sasaki, Junko ^{[2
]}

Omori, Yumio ^{[1
]}

Miyake, Mikiko ^{[1
]}

Morioka, Shin ^{[1
]}

Guo, Ying ^{[1
]}

Sasaki, Takehiko ^{[2
]}

Hazeki, Osamu ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Hiroshima 7348553, Japan

[2] Akita Univ, Dept Pathol & Immunol, Sch Med, Akita 0108543, Japan

来源：

PLOS ONE | 2015年 / 10卷 / 11期

基金：

日本学术振兴会;

关键词：

MYOSIN-X; PHOSPHATIDYLINOSITOL; 3-KINASES; PHOSPHOINOSITIDE; 3-KINASE; ROLES; RAB5; PHOSPHATASES; ENDOCYTOSIS; ACTIVATION;

D O I：

10.1371/journal.pone.0142091

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Phagocytosis is a highly conserved process whereby phagocytic cells engulf pathogens and apoptotic bodies. The present study focused on the role of inositol polyphosphate-4-phosphatase type I (Inpp4a) in phagocytosis. Raw264.7 cells that express shRNA against Inpp4a (shInpp4a cells) showed significantly increased phagocytic activity. The introduction of shRNA-resistant human Inpp4a abolished this increase. Macrophages from Inpp4a knockout mice showed similar increases in the phagocytic activity. Inpp4a was recruited to the phagosome membrane by a mechanism other than the direct interaction with Rab5. PtdIns(3,4)P-2 increased on the phagosome of shInpp4a cells, while PtdIns(3) P significantly decreased. The results indicate that Inpp4a negatively regulates the phagocytic activity of macrophages as a member of the sequential dephosphorylation system that metabolizes phagosomal PtdIns(3,4,5)P-3 to PtdIns(3)P.

引用

页数：18

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