Resorcinol-spectrophotometric method for the determination of fructose in mustard leaf's amylose

被引：0

作者：

Hasenqimeng ^{[1
]}

He, FG ^{[1
]}

机构：

[1] Univ Inner Mongolia Nationalities, Sch Technol, Tonglian 028043, Peoples R China

来源：

SPECTROSCOPY AND SPECTRAL ANALYSIS | 2002年 / 22卷 / 03期

关键词：

mustard leaf of amylose; fructose; content; spectrophotometer;

D O I：

暂无

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A method using seliwanoff reaction is proposed for the determination of fructose in the amylose of mustard leaves. Fructose reacts with resorcinol forming a red compound with a maximum absorption at 473 nm. The calibration curve is linear over the range of 0-83 mug . mL(-1) with a correlation coefficient of 0.999 8. The content of fructose in the amylose of mustard leaves is found to be 11.41%. Quntitative analysis used for the fructose of syrupus include such as volumetric analysis, then layer chromatography, polarimetry, high pressure liquid chromatography and spectrophotometric analysis. According to different color-developing agents, spectrophotometric analysis may be classified as carbazole method, ammonium molybdate way and resorcinol way etc. reported by foreign papers is hard to operate and colored complex is unstable.

引用

页码：446 / 448

页数：3