Kinetic variability and modulation of dSlo, a cloned calcium-dependent potassium channel

被引：11
作者：
Bowlby, MR
Levitan, IB
机构：
[1] BRANDEIS UNIV,DEPT BIOCHEM,WALTHAM,MA 02254
[2] BRANDEIS UNIV,VOLEN CTR COMPLEX SYST,WALTHAM,MA 02254
来源：
NEUROPHARMACOLOGY | 1996年 / 35卷 / 07期
基金：
美国国家卫生研究院;
关键词：
calcium-dependent potassium channel; slopoke; channel modulation; ATP gamma S; protein kinase; Xenopus oocytes; patch clamp;
D O I：
10.1016/0028-3908(96)00090-1
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We have examined, using patch recording, the modulation by ATP gamma S of the cloned Drosophila slopoke calcium-dependent potassium channel (dslo) expressed in Xenopus oocytes. There is a large variation in the gating kinetics, open probability, and conductance level of the Channel in this expression system, which complicates the analysis of modulatory events. Addition of ATP gamma S to the intracellular face of the patch does not consistently alter the overall open probability of dSlo, but it does increase the frequency of appearance of an exceptionally long-lived closed state of the channel. This modulation is not blocked by an inhibitor of several serine/threonine protein kinases, nor by mutation of a serine residue that is a target for phosphorylation by protein kinase A. Thus, ATP gamma S may alter dSlo kinetic properties by some mechanism other than serine/threonine phosphorylation. Copyright (C) 1996 Elsevier Science Ltd
引用
收藏
页码：867 / 875
页数：9
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