ISOLATION OF TOXOPLASMA GONDII FROM ANIMALS IN DURANGO, MEXICO

被引:62
|
作者
Dubey, J. P. [1 ]
Velmurugan, G. V. [1 ]
Alvarado-Esquivel, C. [1 ]
Alvarado-Esquivel, D. [1 ]
Rodriguez-Pena, S. [1 ]
Martinez-Garcia, S. [1 ]
Gonzalez-Herrera, A. [1 ]
Ferreira, L. R. [1 ]
Kwok, O. C. H. [1 ]
Su, C. [1 ]
机构
[1] USDA, Anim Parasit Dis Lab, Anim & Nat Resources Inst, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA
关键词
FREE-RANGE CHICKENS; GENETIC-CHARACTERIZATION; PREVALENCE; DOGS; ANTIBODIES; CATS; INFECTION; AMERICA; BRAZIL; STATE;
D O I
10.1645/GE-1874.1
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Little is known concerning the epidemiology of Toxoplasma gondii infection in people and animal in rural Mexico. Serum samples and tissues from 150 dogs (Canis familaris), 150 cats (Felis catus). 65 opossums (Didelphis virginianus). 249 rats (Rattus spp.). 127 mice (Mus musculus), and 69 squirrels (Spermophilus variegatus) from the Durango area were evaluated for T. gondii infection. Using a modified agglutination test and a serum dilution of 1:25, antibodies to this parasite were found in 68 (45.3%) of 150 dogs. 14 (9.3%) of 150 cats, 11 (16.6%) of 66 opossums, 2 (0.8%) of 249 rats, 4 (3.1%) of 127 mice, and 0 of 69 squirrels. Tissues (brain and heart) of dogs, cats, opossums, rats, mice, and squirrels were bioassayed in mice for the presence of T. gondii. Viable T. gondii was isolated in tissues from 3 of 28 seropositive dogs and 5 of 8 seropositive cats, but not from the other animals. The DNA obtained from the 3 T. gondii isolates from dogs, 6 isolates from 5 cats, and 4 isolates from free-range chickens from Mexico, previously isolate, were genotyped. The PCR-RFLP typing, which used 11 markers (B1, SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358. PKI, and Apico), identified 5 genotypes. One genotype (the 4 chicken isolates) belongs to the clonal Type III lineage, three genotypes were reported in previous reports, and 1 genotype is unique.
引用
收藏
页码:319 / 322
页数:4
相关论文
共 50 条
  • [41] Seroprevalence of Toxoplasma gondii in domestic cats, dogs and rabbits from Poland
    Turlewicz-Podbielska, Hanna
    Ruszkowski, Jakub Jedrzej
    Gogulski, Maciej
    Pomorska-Mol, Malgorzata
    VETERINARY RESEARCH COMMUNICATIONS, 2023, 47 (03) : 1753 - 1758
  • [42] First Detection of Toxoplasma gondii DNA in a Wild Bat from Colombia
    Zamora-Velez, Alejandro
    Cuadrado-Rios, Sebastian
    Hernandez-Pinson, Andres
    Mantilla-Meluk, Hugo
    Enrique Gomez-Marin, Jorge
    ACTA PARASITOLOGICA, 2020, 65 (04) : 969 - 973
  • [43] Isolation and characterization of Toxoplasma gondii strains from stray cats revealed a single genotype in Beijing, China
    Qian, Weifeng
    Wang, Hui
    Su, Chunlei
    Shan, Dan
    Cui, Xia
    Yang, Na
    Lv, Chaochao
    Liu, Qun
    VETERINARY PARASITOLOGY, 2012, 187 (3-4) : 408 - 413
  • [44] Toxoplasma gondii infections in birds, companion, food and recreational animals in Nigeria: A systematic review and meta-analysis
    Karshima, Solomon Ngutor
    Karshima, Magdalene Nguvan
    Karaye, Gloria Pisha
    Oziegbe, Stanley David
    VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS, 2020, 21
  • [45] Seroprevalence of Toxoplasma gondii in Domestic and Wild Animals From the Fernando de Noronha, Brazil
    Costa, D. G. C.
    Marvulo, M. F. V.
    Silva, J. S. A.
    Santana, S. C.
    Magalhaes, F. J. R.
    Lima Filho, C. D. F.
    Ribeiro, V. O.
    Alves, L. C.
    Mota, R. A.
    Dubey, J. P.
    Silva, J. C. R.
    JOURNAL OF PARASITOLOGY, 2012, 98 (03) : 679 - 680
  • [46] Seroprevalence of Toxoplasma gondii in domestic pets from metropolitan regions of Panama
    Rengifo-Herrera, Claudia
    Pile, Edwin
    Garcia, Anabel
    Perez, Alexander
    Perez, Dimas
    Nguyen, Felicia K.
    de la Guardia, Valli
    Mcleod, Rima
    Caballero, Zuleima
    PARASITE, 2017, 24
  • [47] Toxoplasma gondii in animals used for human consumption
    Tenter, Astrid M.
    MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2009, 104 (02): : 364 - 369
  • [48] Risk factors and presence of antibodies to Toxoplasma gondii in dogs from the coast of Sao Paulo State, Brazil
    da Silva, Rodrigo Costa
    de Lima, Vanessa Yuri
    Tanaka, Erika Maemi
    da Silva, Aristeu Vieira
    de Souza, Luiz Carlos
    Langoni, Helio
    PESQUISA VETERINARIA BRASILEIRA, 2010, 30 (02): : 161 - 166
  • [49] Seropositivity of Toxoplasma gondii in domestic donkeys (Equus asinus) and isolation of T. gondii from farm cats
    Dubey, J. P.
    Ness, S. L.
    Kwok, O. C. H.
    Choudhary, S.
    Mittel, L. D.
    Divers, T. J.
    VETERINARY PARASITOLOGY, 2014, 199 (1-2) : 18 - 23
  • [50] High seroprevalence of antibodies to Toxoplasma gondii in wild animals from Portugal
    Lopes, Ana Patricia
    Sargo, Roberto
    Rodrigues, Manuela
    Cardoso, Luis
    PARASITOLOGY RESEARCH, 2011, 108 (05) : 1163 - 1169