Improvements of Vaginal Atrophy without Systemic Side Effects after Topical Application of Pueraria mirifica, a Phytoestrogen-rich Herb, in Postmenopausal Cynomolgus Macaques

被引:10
作者
Jaroenporn, Sukanya [1 ]
Urasopon, Nontakorn [2 ]
Watanabe, Gen [3 ,4 ]
Malaivijitnond, Suchinda [1 ]
机构
[1] Chulalongkorn Univ, Fac Sci, Dept Biol, Primate Res Unit, Bangkok 10330, Thailand
[2] Ubon Ratchathani Univ, Fac Agr, Dept Anim Sci, Ubon Ratchathani 34190, Thailand
[3] Tokyo Univ Agr & Technol, Fac Agr, Dept Vet Med, Lab Vet Physiol, Tokyo 1838509, Japan
[4] Gifu Univ, United Grad Sch Vet Sci, Dept Basic Vet Sci, Gifu 5011193, Japan
关键词
Menopause; Phytoestrogens; Sex skin color; Vaginal dryness; FEMALE RHESUS-MONKEYS; LONG-TAILED MACAQUES; ESTROGENIC ACTIVITY; MENSTRUAL-CYCLE; SKIN COLORATION; SEXUAL-BEHAVIOR; HORMONE; ESTRADIOL; GENISTEIN; WOMEN;
D O I
10.1262/jrd.2013-144
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The estrogenic efficacy of topical vaginal application of Pueraria mirifica extract (PM) on the restoration of vaginal atrophy, and the presence of any systemic side effects, were investigated in postmenopausal cynomolgus macaques. Twelve postmenopausal cynomolgus macaques, with complete cessation of menstruation for at least 5 years before start of this experiment, were divided into three groups. They received a topical vaginal application daily of 0.1 or 1% (w/w) PM cream or a conjugated equine estrogen (CEE) cream (a mixture of estrone, equilin, 17 beta-dihydroequilin, 17 alpha-estradiol and 17 alpha-dihydroequilin at 0.625 mg total estrogen/g cream) for 28 days. Estrogenic efficacy was assessed weekly by vaginal cytology assay and vaginal pH measurement, whilst the plasma luteinizing hormone (LH) and sex skin coloration levels were determined at the end of each treatment period to evaluate the systemic side effects. PM significantly increased the proportion of superficial cells in a dose-dependent manner, with a similar efficacy between 1% (w/w) PM and CEE. Together with increased vaginal maturation, PM decreased the vaginal pH to acidic levels, as observed in the GEE group. PM induced no detected systemic side effects, whilst GEE decreased the plasma LH level and increased the reddish color of the sex skin during the posttreatment period. Topical vaginal treatment with PM stimulated the maturation of the vaginal epithelium without causing systemic side effects in postmenopausal monkeys. The implication is that PM could be a safer alternative to treat vaginal atrophy in postmenopausal women.
引用
收藏
页码:238 / 245
页数:8
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