Baicalein attenuates proteasome inhibition-induced apoptosis by suppressing the activation of the mitochondrial pathway and the caspase-8-and Bid-dependent pathways

被引:28
|
作者
Jung, Eun Byul [1 ]
Lee, Chung Soo [1 ]
机构
[1] Chung Ang Univ, Dept Pharmacol, Coll Med, Seoul 156756, South Korea
关键词
Proteasome inhibition; Baicalein; Differentiated PC12 cells; Apoptosis-relatecl proteins; Protection; CELL-DEATH; PC12; CELLS; OXIDATIVE STRESS; NEURODEGENERATIVE DISEASES; SIGNALING PATHWAYS; IN-VITRO; GLUTATHIONE; GROWTH; SCUTELLARIA; PROTECTS;
D O I
10.1016/j.ejphar.2014.02.039
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Impairment in ubiquitin-proteasome system has been shown to be implicated in the pathogenesis in neurodegenerative disorders, such as Parkinson's disease. Flavonoid baicalein has demonstrated antioxidant and anti-inflammatory effects. However, the effect of baicalein on the neuronal cell death due to proteasome inhibition has not been studied. Thus, in the respect of the cell death process, we assessed the effect of baicalein on the proteasome inhibition-induced apoptosis using differentiated PC12 cells. The proteasome inhibitors MG132 and MG115 induced a decrease in Bid, Bcl-2, Bcl-xL and survivin protein levels, an increase in Bax levels, loss of the mitochondrial transmembrane potential, release of cytochrome c, activation of caspases (-8, -9 and -3), an increase in the tumor suppressor p53 levels and cleavage of PARP-1. Baicalein attenuated the proteasome inhibition-induced changes in the levels of apoptosis-related proteins, formation of reactive oxygen species, depletion of GSH, DNA damage and cell death. The results show that baicalein may attenuate the proteasome inhibition-induced apoptosis in PC12 cells by suppressing the activation of the mitochondrial pathway and the caspase-8- and Bid-dependent pathways. The preventive effect appears to be attributed to its inhibitory effect on the formation of reactive oxygen species and depletion of GSH. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:116 / 124
页数:9
相关论文
共 46 条
  • [1] Lamotrigine Attenuates Proteasome Inhibition-Induced Apoptosis by Suppressing the Activation of the Mitochondrial Pathway and the Caspase-8-and Bid-Dependent Pathways
    Nam, Yoon Jeong
    Kim, Arum
    Lee, Min Sung
    Shin, Yong Kyoo
    Sohn, Dong Suep
    Lee, Chung Soo
    NEUROCHEMICAL RESEARCH, 2016, 41 (10) : 2503 - 2516
  • [2] Lamotrigine Attenuates Proteasome Inhibition-Induced Apoptosis by Suppressing the Activation of the Mitochondrial Pathway and the Caspase-8- and Bid-Dependent Pathways
    Yoon Jeong Nam
    Arum Kim
    Min Sung Lee
    Yong Kyoo Shin
    Dong Suep Sohn
    Chung Soo Lee
    Neurochemical Research, 2016, 41 : 2503 - 2516
  • [3] Rotundarpene attenuates cholesterol oxidation product-induced apoptosis by suppressing the mitochondrial pathway and the caspase-8-and bid-dependent pathways
    Lee, Da Hee
    Nam, Yoon Jeong
    Lee, Min Sung
    Sohn, Dong Suep
    Lee, Chung Soo
    EUROPEAN JOURNAL OF PHARMACOLOGY, 2015, 749 : 39 - 48
  • [4] Casein kinase 2 inhibition attenuates cholesterol oxidation product-induced apoptosis by suppressing the activation of the mitochondrial pathway and the caspase-8-and bid-dependent pathways
    Jung, Eun Byul
    Kim, Yun Jeong
    Lee, Chung Soo
    NEUROCHEMISTRY INTERNATIONAL, 2014, 65 : 30 - 39
  • [5] Rotundarpene prevents TRAIL-induced apoptosis in human keratinocytes by suppressing the caspase-8-and Bid-pathways and the mitochondrial pathway
    Lee, Da Hee
    Nam, Yoon Jeong
    Kim, Yun Jeong
    Lee, Min Won
    Lee, Chung Soo
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2014, 387 (12) : 1209 - 1219
  • [6] Rotundarpene prevents TRAIL-induced apoptosis in human keratinocytes by suppressing the caspase-8- and Bid-pathways and the mitochondrial pathway
    Da Hee Lee
    Yoon Jeong Nam
    Yun Jeong Kim
    Min Won Lee
    Chung Soo Lee
    Naunyn-Schmiedeberg's Archives of Pharmacology, 2014, 387 : 1209 - 1219
  • [7] Quercetin-3-O-(2"-galloyl)-α-L-rhamnopyranoside prevents TRAIL-induced apoptosis in human keratinocytes by suppressing the caspase-8-and Bid-pathways and the mitochondrial pathway
    Kim, Yun Jeong
    Jung, Eun Byul
    Seo, Seong Jun
    Park, Kwan Hee
    Lee, Min Won
    Lee, Chung Soo
    CHEMICO-BIOLOGICAL INTERACTIONS, 2013, 204 (03) : 144 - 152
  • [8] Relief of extrinsic pathway inhibition by the bid-dependent mitochondrial release of smac in fas-mediated hepatocyte apoptosis
    Li, SC
    Zhao, YG
    He, X
    Kim, TH
    Kuharsky, DK
    Rabinowich, H
    Chen, J
    Du, CY
    Yin, XM
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (30) : 26912 - 26920
  • [9] Flavanonol Taxifolin Attenuates Proteasome Inhibition-Induced Apoptosis in Differentiated PC12 Cells by Suppressing Cell Death Process
    Yoon Jeong Nam
    Da Hee Lee
    Yong Kyoo Shin
    Dong Suep Sohn
    Chung Soo Lee
    Neurochemical Research, 2015, 40 : 480 - 491
  • [10] Flavanonol Taxifolin Attenuates Proteasome Inhibition-Induced Apoptosis in Differentiated PC12 Cells by Suppressing Cell Death Process
    Nam, Yoon Jeong
    Lee, Da Hee
    Shin, Yong Kyoo
    Sohn, Dong Suep
    Lee, Chung Soo
    NEUROCHEMICAL RESEARCH, 2015, 40 (03) : 480 - 491