Chemical Methods for Selective Labeling of Proteins

被引：46

作者：

Rawale, Dattatraya G. ^{[1
]}

Thakur, Kalyani ^{[1
]}

Adusumalli, Srinivasa Rao ^{[1
]}

Rai, Vishal ^{[1
]}

机构：

[1] Indian Inst Sci Educ & Res Bhopal, Dept Chem, Bhopal Bypass Rd, Bhopal 462066, Madhya Pradesh, India

来源：

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY | 2019年 / 2019卷 / 40期

关键词：

Chemoselectivity; Site-selectivity; Residue specificity; Bioconjugation; Proteins; NEWLY SYNTHESIZED PROTEINS; SITE-SPECIFIC PEGYLATION; UNNATURAL AMINO-ACIDS; NATIVE PROTEINS; RHODIUM CARBENOIDS; HISTIDINE-RESIDUES; ORGANIC-CHEMISTRY; MEDIATED PROTEIN; CYSTEINE; BIOCONJUGATION;

D O I：

10.1002/ejoc.201900801

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Protein bioconjugation poses outstanding questions of selectivity to the organic transformations. Besides, the presence of a pool of functional groups in the structural outfit of a protein brings its own set of characteristics. In this minireview, we highlight the challenges faced by a chemical transformation to deliver selectivity in the modification of proteins. The examples of pre-engineered proteins outline the attributes associated with chemoselectivity and chemical orthogonality. Building on this foundation, we discuss the complexity of site-selectivity in the single-site modification of native proteins. The gradual evolution of chemical methods while addressing the challenges associated with different amino acids are outlined. The modular methods for labeling a residue independent of its reactivity order closes the discussion.

引用

页码：6749 / 6763

页数：15

共 183 条

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