Capsular polysaccharide produced by the thermophilic cyanobacterium Mastigocladus laminosus -: Structural study of an undecasaccharide obtained by lithium degradation

被引：15
作者：
Gloaguen, V
Morvan, H
Hoffmann, L
Plancke, Y
Wieruszeski, JM
Lippens, G
Strecker, G
机构：
[1] Univ Limoges, Inst Biotechnol, Equipe Glycobiol Vegetale, F-87060 Limoges, France
[2] CRP CTr Univ Luxembourg, Luxembourg, Luxembourg
[3] Univ Sci & Tech Lille Flandres Artois, Chim Biol Lab, CNRS, UMR 111, F-59655 Villeneuve Dascq, France
[4] Inst Pasteur, CNRS, URA 1309, F-59019 Lille, France
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1999年 / 266卷 / 03期
关键词：
cyanobacterium; exopolysaccharide; lithium degradation; NMR; structure;
D O I：
10.1046/j.1432-1327.1999.00879.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The capsular polysaccharide produced by the thermophilic cyanobacterium Mastigocladus laminosus has been subjected to a specific degradation with lithium in ethylenediamine. The released undecasaccharide attached to one unit of tetrahydroxycyclopentanecarboxylic acid has been characterized by a combination of 2D nuclear magnetic resonance spectroscopy, mass spectrometry, monosaccharidic composition and linkage analyses. From the overlap of the structure of this oligosaccharide with previously identified di-, tri- and pentasaccharides released by mild acid hydrolysis, the capsular polysaccharide was deduced to have a pentadecasaccharide repeating unit with the following structure: [GRAPHICS]
引用
收藏
页码：762 / 770
页数：9
相关论文
共 30 条
[1]   CHARACTERIZATION OF MACROMOLECULAR FLOCCULANTS PRODUCED BY PHORMIDIUM SP STRAIN-J-1 AND BY ANABAENOPSIS-CIRCULARIS PCC-6720 [J].
BAROR, Y ;
SHILO, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (09) :2226-2230
[2]   POLYSACCHARIDES FROM CYANOBACTERIA .1. [J].
BERTOCCHI, C ;
NAVARINI, L ;
CESARO, A ;
ANASTASIO, M .
CARBOHYDRATE POLYMERS, 1990, 12 (02) :127-153
[3]   NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS [J].
BLUMENKR.N ;
ASBOEHAN.G .
ANALYTICAL BIOCHEMISTRY, 1973, 54 (02) :484-489
[4]   C-13 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY OF MONOSACCHARIDES [J].
BOCK, K ;
PEDERSEN, C .
ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1983, 41 :27-66
[5]   Structural elucidation of a novel exopolysaccharide produced by a mucoid clinical isolate of Burkholderia cepacia -: Characterization of a trisubstituted glucuronic acid residue in a heptasaccharide repeating unit [J].
Cérantola, S ;
Lemassu-Jacquier, A ;
Montrozier, H .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 260 (02) :373-383
[6]   POLYSACCHARIDES FROM CYANOBACTERIA .5. PHYSICOCHEMICAL PROPERTIES OF THE EXOCELLULAR POLYSACCHARIDE FROM CYANOSPIRA-CAPSULATA [J].
CESARO, A ;
LIUT, G ;
BERTOCCHI, C ;
NAVARINI, L ;
URBANI, R .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1990, 12 (02) :79-84
[7]   SAMARIUM(II) IODIDE PROMOTED RING CONTRACTION OF CARBOHYDRATE-DERIVATIVES - AN EXPEDITIOUS SYNTHESIS OF FUNCTIONALIZED CYCLOPENTANES [J].
CHENEDE, A ;
POTHIER, P ;
SOLLOGOUB, M ;
FAIRBANKS, AJ ;
SINAY, P .
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1995, (13) :1373-1374
[8]   Exocellular polysaccharides from cyanobacteria and their possible applications [J].
De Philippis, R ;
Vincenzini, M .
FEMS MICROBIOLOGY REVIEWS, 1998, 22 (03) :151-175
[9]   COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].
DUBOIS, M ;
GILLES, KA ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356
[10]   PRODUCTION, ISOLATION AND PRELIMINARY CHARACTERIZATION OF THE EXOPOLYSACCHARIDE OF THE CYANOBACTERIUM SPIRULINA-PLATENSIS [J].
FILALIMOUHIM, R ;
CORNET, JF ;
FONTAINE, T ;
FOURNET, B ;
DUBERTRET, G .
BIOTECHNOLOGY LETTERS, 1993, 15 (06) :567-572
123