Enzymatic synthesis of palm-based ascorbyl esters

被引：39

作者：

Burham, Hamidah ^{[1
]}

Gafoor, Raizatul Ainaa ^{[1
]}

Rasheed, Abdul ^{[1
]}

Noor, Noorullhamezon Md ^{[1
]}

Badruddin, Suzaini ^{[1
]}

Sidek, Hamidah ^{[1
]}

机构：

[1] Sirim Berhad, Environm & Bioproc Technol Ctr, Shah Alam 40911, Selangor, Malaysia

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2009年 / 58卷 / 1-4期

关键词：

Antioxidant; Palm-based ascorbyl esters; Palm oil; Novozyme; 435; IMMOBILIZED LIPASE; ACID;

D O I：

10.1016/j.molcatb.2008.12.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of palm-based ascorbyl esters through transesterification of ascorbic acid and palm oil in tert-amyl alcohol catalyzed by immobilized lipase is described. Highest conversion (70-75%) was determined after 16h reaction at 40 degrees C using lipase (Novozyme 435 from Candida antartica) with an ascorbic acid to palm oil mole ratio of 1:8. The purified product was further characterized by C-13 NMR and GC-MS and the mixture of ascorbyl monoesters obtained were identified as ascorbyl monoolleate (61%). ascorbyl monopalmitate (30%) and ascorbyl monostearate (9%). The antioxidant activity of palm-based ascorbyl esters was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. The results showed that pure palm-based ascorbyl esters have an antioxidant activity with an IC50 value of 0.1 mg/mL. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：153 / 157

页数：5

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