Enzymatic synthesis of palm-based ascorbyl esters

被引：39

作者：

Burham, Hamidah ^{[1
]}

Gafoor, Raizatul Ainaa ^{[1
]}

Rasheed, Abdul ^{[1
]}

Noor, Noorullhamezon Md ^{[1
]}

Badruddin, Suzaini ^{[1
]}

Sidek, Hamidah ^{[1
]}

机构：

[1] Sirim Berhad, Environm & Bioproc Technol Ctr, Shah Alam 40911, Selangor, Malaysia

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2009年 / 58卷 / 1-4期

关键词：

Antioxidant; Palm-based ascorbyl esters; Palm oil; Novozyme; 435; IMMOBILIZED LIPASE; ACID;

D O I：

10.1016/j.molcatb.2008.12.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of palm-based ascorbyl esters through transesterification of ascorbic acid and palm oil in tert-amyl alcohol catalyzed by immobilized lipase is described. Highest conversion (70-75%) was determined after 16h reaction at 40 degrees C using lipase (Novozyme 435 from Candida antartica) with an ascorbic acid to palm oil mole ratio of 1:8. The purified product was further characterized by C-13 NMR and GC-MS and the mixture of ascorbyl monoesters obtained were identified as ascorbyl monoolleate (61%). ascorbyl monopalmitate (30%) and ascorbyl monostearate (9%). The antioxidant activity of palm-based ascorbyl esters was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. The results showed that pure palm-based ascorbyl esters have an antioxidant activity with an IC50 value of 0.1 mg/mL. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：153 / 157

页数：5

共 17 条

[1]

CHO F, 1993, ELAEIS, V6, P25

[2] Zeolite molecular sieves have dramatic acid-base effects on enzymes in nonaqueous media [J].

Fontes, N ;

Partridge, J ;

Halling, PJ ;

Barreiros, S .

BIOTECHNOLOGY AND BIOENGINEERING, 2002, 77 (03) :296-305

[3] Free radical-scavenging activity of Taiwanese native plants [J].

Hou, WC ;

Lin, RD ;

Cheng, KT ;

Hung, YT ;

Cho, CH ;

Chen, CH ;

Hwang, SY ;

Lee, MH .

PHYTOMEDICINE, 2003, 10 (2-3) :170-175

[4] Production of ascorbyl palmitate by surfactant-coated lipase in organic media [J].

Hsieh, Hsin-Ju ;

Nair, Giridhar R. ;

Wu, Wen-Teng .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (16) :5777-5781

[5] Enzymatic synthesis of fatty acid ascorbyl esters [J].

Humeau, C ;

Girardin, M ;

Rovel, B ;

Miclo, A .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1998, 5 (1-4) :19-23

[6] Synthesis of 6-O-unsaturated acyl L-ascorbates by immobilized lipase in acetone in the presence of molecular sieve [J].

Kuwabara, K ;

Watanabe, Y ;

Adachi, S ;

Nakanishi, K ;

Matsuno, R .

BIOCHEMICAL ENGINEERING JOURNAL, 2003, 16 (01) :17-22

[7]

MALCATA FX, 1990, J AM OIL CHEM SOC, V67, P890, DOI 10.1007/BF02541845

[8] Study of vitamin ester synthesis by lipase-catalyzed transesterification in organic media [J].

Maugard, T ;

Tudella, J ;

Legoy, MD .

BIOTECHNOLOGY PROGRESS, 2000, 16 (03) :358-362

[9]

*MERCK KGAA, DRY AG

[10] PROCESS CONSIDERATIONS OF CONTINUOUS FAT MODIFICATION WITH AN IMMOBILIZED LIPASE [J].

POSORSKE, LH ;

LEFEBVRE, GK ;

MILLER, CA ;

HANSEN, TT ;

GLENVIG, BL .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1988, 65 (06) :922-926

← 1 2 →