C. elegans in high-throughput drug discovery

被引：184
作者：
O'Reilly, Linda P. [1 ]
Luke, Cliff J. [1 ]
Perlmutter, David H. [1 ]
Silverman, Gary A. [1 ]
Pak, Stephen C. [1 ]
机构：
[1] Univ Pittsburgh, Childrens Hosp Pittsburgh UPMC, Sch Med, Dept Pediat Cell Biol & Physiol, Pittsburgh, PA 15224 USA
来源：
ADVANCED DRUG DELIVERY REVIEWS | 2014年 / 69卷
关键词：
Whole organism model; High-throughput screening; Small molecules; Pharmaceuticals; Drug target identification; Phenotypic analysis; Human disease models; CAENORHABDITIS-ELEGANS; LIFE-SPAN; ALPHA-1-ANTITRYPSIN DEFICIENCY; BEHAVIORAL-ANALYSIS; PARKINSONS-DISEASE; MODEL; GENES; IDENTIFICATION; DYSTROPHIN; LONGEVITY;
D O I：
10.1016/j.addr.2013.12.001
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Caenorhabditis elegans has been proven to be a useful model organism for investigating molecular and cellular aspects of numerous human diseases. More recently, investigators have explored the use of this organism as a tool for drug discovery. Although earlier drug screens were labor-intensive and low in throughput, recent advances in high-throughput liquid workflows, imaging platforms and data analysis software have made C elegans a viable option for automated high-throughput drug screens. This review will outline the evolution of C elegans-based drug screening, discuss the inherent challenges of using C elegans, and highlight recent technological advances that have paved the way for future drug screens. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：247 / 253
页数：7
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