The β-Subunit of Cholera Toxin has a High Affinity for Ganglioside GM1 Embedded into Solid Supported Lipid Membranes with a Lipid Raft-Like Composition

被引：20

作者：

Margheri, G. ^{[1
]}

D'Agostino, R. ^{[2
]}

Trigari, S. ^{[1
]}

Sottini, S. ^{[1
]}

Del Rosso, M. ^{[2
]}

机构：

[1] CNR, Inst Complex Syst, I-50019 Florence, Italy

[2] Univ Florence, Dept Pathol & Expt Oncol, I-50134 Florence, Italy

来源：

LIPIDS | 2014年 / 49卷 / 02期

关键词：

Lipid rafts; Gangliosides; Surface plasmon resonance; PROTEIN; BINDING; SURFACE; CAVEOLAE;

D O I：

10.1007/s11745-013-3845-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this communication, we report on the fabrication of GM1-rich solid-supported bilayer lipid membranes (ssBLM) made of sphingomyelin and cholesterol, the main components of lipid rafts, which are the physiological hosting microenvironment of GM1 on the cell membrane. The functionality of the ganglioside has been checked by measuring the apparent dissociation constant K (D) of the complex formed by the beta-subunit of the cholera toxin and GM1. The value found deviates less than one order of magnitude from that measured for in vivo cells, indicating the potential of these ssBLM as optimized in vitro biomimetic platforms.

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收藏

页码：203 / 206

页数：4

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