New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses

被引：26

作者：

Naguib, Mahmoud M. ^{[1
,2
]}

El-Kady, Magdy F. ^{[3
]}

Lueschow, Dorte ^{[4
]}

Hassan, Kareem E. ^{[3
]}

Arafa, Abdel-Satar ^{[2
]}

El-Zanaty, Ali ^{[2
]}

Hassan, Mohamed K. ^{[2
]}

Hafez, Hafez M. ^{[4
]}

Grund, Christian ^{[1
]}

Harder, Timm C. ^{[1
]}

机构：

[1] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Suedufer 10, D-17493 Greifswald Insel Riems, Germany

[2] Anim Hlth Res Inst, Natl Lab Vet Qual Control Poultry Prod, POB 246, Giza 12618, Egypt

[3] Beni Suef Univ, Fac Vet Med, Poultry Dis Dept, Bani Suwayf 62511, Egypt

[4] Free Univ Berlin, Inst Poultry Dis, D-14163 Berlin, Germany

来源：

JOURNAL OF VIROLOGICAL METHODS | 2017年 / 245卷

关键词：

AMINO-ACIDS; RECOMBINATION; SEROTYPE; STRAIN; ASSAY; IDENTIFICATION; CORONAVIRUSES; POULTRY; BROILER; RNA;

D O I：

10.1016/j.jviromet.2017.02.018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：19 / 27

页数：9

共 45 条

[1]

Abdel-Moneim A.S., 2002, Vet. Med. J. Giza, V50, P1065

[2] SI gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt [J].

Abdel-Moneim, Ahmed S. ;

El-Kady, Magdy F. ;

Ladman, Brian S. ;

Gelb, Jack, Jr. .

VIROLOGY JOURNAL, 2006, 3 (1)

[3] Emergence of a novel genotype of avian infectious bronchitis virus in Egypt [J].

Abdel-Moneim, Ahmed S. ;

Afifi, Manal A. ;

El-Kady, Magdy F. .

ARCHIVES OF VIROLOGY, 2012, 157 (12) :2453-2457

[4] Molecular analysis of the 793/B serotype of infectious bronchitis virus in Great Britain [J].

Adzhar, A ;

Gough, RE ;

Haydon, D ;

Shaw, K ;

Britton, P ;

Cavanagh, D .

AVIAN PATHOLOGY, 1997, 26 (03) :625-640

[5] Rapid pathotyping of Newcastle disease virus (NDV) using fluorogenic probes in a PCR assay [J].

Aldous, EW ;

Collins, MS ;

McGoldrick, A ;

Alexander, DJ .

VETERINARY MICROBIOLOGY, 2001, 80 (03) :201-212

[6] Mechanisms of Coronavirus Cell Entry Mediated by the Viral Spike Protein [J].

Belouzard, Sandrine ;

Millet, Jean K. ;

Licitra, Beth N. ;

Whittaker, Gary R. .

VIRUSES-BASEL, 2012, 4 (06) :1011-1033

[7] Ultrafast Approximation for Phylogenetic Bootstrap [J].

Bui Quang Minh ;

Minh Anh Thi Nguyen ;

von Haeseler, Arndt .

MOLECULAR BIOLOGY AND EVOLUTION, 2013, 30 (05) :1188-1195

[8] Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens [J].

Callison, Scott A. ;

Hilt, Deborah A. ;

Boynton, Tye O. ;

Sample, Brenda F. ;

Robison, Robert ;

Swayne, David E. ;

Jackwood, Mark W. .

JOURNAL OF VIROLOGICAL METHODS, 2006, 138 (1-2) :60-65

[9] Coronaviruses in poultry and other birds [J].

Cavanagh, D .

AVIAN PATHOLOGY, 2005, 34 (06) :439-448

[10] INFECTIOUS-BRONCHITIS VIRUS - EVIDENCE FOR RECOMBINATION WITHIN THE MASSACHUSETTS SEROTYPE [J].

CAVANAGH, D ;

DAVIS, PJ ;

COOK, JKA .

AVIAN PATHOLOGY, 1992, 21 (03) :401-408

← 1 2 3 4 5 →