New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses

被引：25

作者：

Naguib, Mahmoud M. ^{[1
,2
]}

El-Kady, Magdy F. ^{[3
]}

Lueschow, Dorte ^{[4
]}

Hassan, Kareem E. ^{[3
]}

Arafa, Abdel-Satar ^{[2
]}

El-Zanaty, Ali ^{[2
]}

Hassan, Mohamed K. ^{[2
]}

Hafez, Hafez M. ^{[4
]}

Grund, Christian ^{[1
]}

Harder, Timm C. ^{[1
]}

机构：

[1] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Suedufer 10, D-17493 Greifswald Insel Riems, Germany

[2] Anim Hlth Res Inst, Natl Lab Vet Qual Control Poultry Prod, POB 246, Giza 12618, Egypt

[3] Beni Suef Univ, Fac Vet Med, Poultry Dis Dept, Bani Suwayf 62511, Egypt

[4] Free Univ Berlin, Inst Poultry Dis, D-14163 Berlin, Germany

来源：

JOURNAL OF VIROLOGICAL METHODS | 2017年 / 245卷

关键词：

AMINO-ACIDS; RECOMBINATION; SEROTYPE; STRAIN; ASSAY; IDENTIFICATION; CORONAVIRUSES; POULTRY; BROILER; RNA;

D O I：

10.1016/j.jviromet.2017.02.018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：19 / 27

页数：9

共 45 条

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