Crystallization and preliminary X-ray analysis of γ-hexachlorocyclohexane dehydrochlorinase LinA from Sphingobium japonicum UT26

被引：4

作者：

Okai, Masahiko ^{[1
]}

Kubota, Keiko ^{[1
]}

Fukuda, Masao ^{[2
]}

Nagata, Yuji ^{[3
]}

Nagata, Koji ^{[1
]}

Tanokura, Masaru ^{[1
]}

机构：

[1] Univ Tokyo, Dept Appl Biol Chem, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[2] Nagaoka Univ Technol, Dept Bioengn, Niigata 9402188, Japan

[3] Tohoku Univ, Grad Sch Life Sci, Dept Environm Life Sci, Sendai, Miyagi 9808577, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2009年 / 65卷

关键词：

SPHINGOMONAS-PAUCIMOBILIS UT26; PSEUDOMONAS-PAUCIMOBILIS; CRYSTAL-STRUCTURE; DEGRADATION; GENES; IDENTIFICATION; BACTERIA; CLONING;

D O I：

10.1107/S1744309109026645

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

LinA from Sphingobium japonicum UT26 catalyzes two steps of dehydrochlorination from gamma-hexachlorocyclohexane (gamma-HCH) to 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN) via gamma-pentachlorocyclohexene (gamma-PCCH). LinA was crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystals belonged to space group P4(1) or P4(3), with unit-cell parameters a = b = 68.9, c = 101.9 angstrom, and diffracted X-rays to 2.25 angstrom resolution. The crystal contained three molecules in the asymmetric unit.

引用

页码：822 / 824

页数：3

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