Bio-Engineered Copper Oxide Nanoparticles Using Citrus Aurantifolia Enzyme Extract and its Anticancer Activity

Copper oxide (CuO) NPs offer promising applications and green synthesis is widely preferred over synthetic methods because of its merits. The past literatures show the preparation of copper nanoparticles from plants extract of leaves but not from the enzymes. But here the greener method of CuO nanoparticles which has been derived from enzymes extract of Citrus aurantifolia is reported. When the enzyme powder obtained from leaf extracts of Citrus aurantifolia was treated with copper sulphate, copper oxide nanoparticles were produced. Such nanoparticles prepared are characterized by UV- Vis, FT-IR, XRD, HR-TEM and scanning electron microscope studies. On the other hand, antibacterial activity against K. pneumonia and S. aureus, was evaluated. Results showed sensibility of K. pneumonia and S. aureus and the antibacterial property improves infections due to drug-resistant bacteria's.