DEAE-dextran hydrochloride behaviour in aqueous solution-The effect of ionic strength and concentration

被引：7
作者：
Kalina, Michal [1 ,2 ]
Kargerova, Andrea [1 ,2 ]
Pekar, Miloslav [1 ,2 ]
机构：
[1] Brno Univ Technol, Fac Chem, Mat Res Ctr, Purkynova 118, Brno 61200, Czech Republic
[2] Inst Phys & Appl Chem, Purkynova 118, Brno 61200, Czech Republic
来源：
CARBOHYDRATE POLYMERS | 2019年 / 220卷
关键词：
DEAE-dextran hydrochloride; Conformation; Ionic strength; Polyelectrolyte; Density model; DYNAMIC LIGHT-SCATTERING; POLYELECTROLYTE; TEMPERATURE; HYALURONAN; ADSORPTION; STIFFNESS; POLYMERS; WATER; SIZE; SALT;
D O I：
10.1016/j.carbpol.2019.05.070
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
DEAE-dextran hydrochloride is a positively charged biocompatible polyelectrolyte. Its behaviour in aqueous solutions - the changes in its colloidal characteristics and resulting conformation changes -were investigated using a combination of light scattering, densitometry and ultrasound spectrometry. The results indicated the formation of a voluminous, hydrated and coiled conformation of chains having average particle sizes in the range of units of microns at low ionic strength. This phenomenon was supported by an increase in DEAE-dextran hydrochloride concentration in the solution. The increase in ionic strength caused the shielding of DEAE-dextran hydrochloride charges on its chain, resulting in the destabilization of the conformation arrangement of the polyelectrolyte and the formation of denser, more compact and stiffer structures having smaller particle sizes. The proposed model of DEAE-dextran hydrochloride density in the studied environments (distilled water, NaCl solutions) showed the possibility of using a single equation model to calculate the overall density of the system.
引用
收藏
页码：163 / 169
页数：7
相关论文
共 36 条
[1]   A novel method to estimate the stiffness of carbohydrate polyelectrolyte polymers based on the ionic strength dependence of zeta potential [J].
Abodinar, Atiga ;
Smith, Alan M. ;
Morris, Gordon A. .
CARBOHYDRATE POLYMERS, 2014, 112 :6-9
[2]  
[Anonymous], 2011, LIGHT SCATTERING SIZ
[3]   Protein adsorption onto diethylaminoethyl dextran modified anion exchanger: Effect of ionic strength and column behavior [J].
Bai, Shu ;
Gong, Lingli ;
Han, Detao ;
Li, Yutong ;
Yu, Linling ;
Sun, Yan .
CHINESE JOURNAL OF CHEMICAL ENGINEERING, 2018, 26 (02) :259-267
[4]   HYDRATION OF NUCLEIC BASES IN DILUTE AQUEOUS-SOLUTIONS - APPARENT MOLAR ADIABATIC AND ISOTHERMAL COMPRESSIBILITIES, APPARENT MOLAR VOLUMES AND THEIR TEMPERATURE SLOPES AT 25-DEGREES-C [J].
BUCKIN, VA .
BIOPHYSICAL CHEMISTRY, 1988, 29 (03) :283-292
[5]   Roles of dextrans on improving lymphatic drainage for liposomal drug delivery system [J].
Feng, Linglin ;
Zhang, Lei ;
Liu, Min ;
Yan, Zhiqiang ;
Wang, Chenyu ;
Gu, Bing ;
Liu, Yu ;
Wei, Gang ;
Zhong, Gaoren ;
Lu, Weiyue .
JOURNAL OF DRUG TARGETING, 2010, 18 (03) :168-178
[6]   Polyelectrolyte complex formation between iota-carrageenan and poly(L-lysine) in dilute aqueous solutions:: a spectroscopic and conformational study [J].
Girod, S ;
Boissère, M ;
Longchambon, K ;
Begu, S ;
Tourne-Pétheil, C ;
Devoisselle, JM .
CARBOHYDRATE POLYMERS, 2004, 55 (01) :37-45
[7]   Densitometry and ultrasound velocimetry of hyaluronan solutions in water and in sodium chloride solution [J].
Kargerova, A. ;
Pekar, M. .
CARBOHYDRATE POLYMERS, 2014, 106 :453-459
[8]   High-Resolution Ultrasonic Spectroscopy Study of Interactions between Hyaluronan and Cationic Surfactants [J].
Kargerova, Andrea ;
Pekar, Miloslav .
LANGMUIR, 2014, 30 (40) :11866-11872
[9]  
Lapasin R., 1995, RHEOLOGY IND POLYSAC, DOI DOI 10.1007/978-1-4615-2185-3
[10]   Formation of polyelectrolyte complexes with diethylaminoethyl dextran: Charge ratio and molar mass effect [J].
Le Cerf, Didier ;
Pepin, Anne Sophie ;
Niang, Pape Momar ;
Cristea, Mariana ;
Karakasyan-Dia, Carole ;
Picton, Luc .
CARBOHYDRATE POLYMERS, 2014, 113 :217-224
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