A simple aptamer biosensor for Salmonellae enteritidis based on fluorescence-switch signaling graphene oxide

被引：20

作者：

Wu, Wei ^{[1
]}

Fang, Zhiyuan ^{[1
,3
]}

Zhao, Shiming ^{[1
,2
]}

Lu, Xuewen ^{[1
]}

Yu, Luxin ^{[1
]}

Mei, Ting ^{[1
]}

Zeng, Lingwen ^{[1
]}

机构：

[1] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Guangzhou 510530, Guangdong, Peoples R China

[2] Anhui Univ, Coll Life Sci & Technol, Hefei 230039, Peoples R China

[3] Guangzhou Med Univ, Affiliated Tumor Hosp, Guangzhou 510095, Guangdong, Peoples R China

来源：

RSC ADVANCES | 2014年 / 4卷 / 42期

关键词：

DNA; PLATFORM; PCR;

D O I：

10.1039/c4ra01901f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this communication, we report a rapid and cost-efficient assay for the detection of S. enteritidis. Specific aptamers with fluorescence labeled S. enteritidis are absorbed into graphene oxide, and the fluorescence is quenched owing to the fluorescence quenching ability of graphene oxide. In the presence of S. enteritidis, the aptamer would release from the graphene oxide to obtain a significant fluorescence recovery. This aptasensor can detect as low as 40 CFU mL(-1) of S. enteritidis in 30 min, and can fulfill the demand of multiplex detection. The cost-effective, rapid and simple aptasensor offers a promising method for food-borne pathogen monitoring during food processing.

引用

收藏

页码：22009 / 22012

页数：4

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