Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae

被引:37
作者
Vest, Katherine E. [1 ]
Wang, Jing [1 ]
Gammon, Micah G. [1 ]
Maynard, Margaret K. [1 ]
White, Olivia L. [2 ]
Cobine, Jai A. [1 ]
Mahone, Wilkerson K. [1 ]
Cobine, Paul A. [1 ]
机构
[1] Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA
[2] Coll Charleston, Charleston, SC 29424 USA
来源
OPEN BIOLOGY | 2016年 / 6卷 / 01期
基金
美国国家科学基金会;
关键词
copper; iron; cytochrome c oxidase; mitochondrial carrier family; CYTOCHROME-C-OXIDASE; SUPEROXIDE-DISMUTASE; LACTOCOCCUS-LACTIS; FLUORESCENT SENSOR; PHYSIOLOGICAL-ROLE; MEMBRANE-PROTEINS; CELLULAR COPPER; YEAST; TRANSPORT; SCO1;
D O I
10.1098/rsob.150223
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In Saccharomyces cerevisiae, the mitochondrial carrier family protein Pic2 imports copper into the matrix. Deletion of PIC2 causes defects in mitochondrial copper uptake and copper-dependent growth phenotypes owing to decreased cytochrome c oxidase activity. However, copper import is not completely eliminated in this mutant, so alternative transport systems must exist. Deletion of MRS3, a component of the iron import machinery, also causes a copper-dependent growth defect on non-fermentable carbon. Deletion of both PIC2 and MRS3 led to a more severe respiratory growth defect than either individual mutant. In addition, MRS3 expressed from a high copy number vector was able to suppress the oxygen consumption and copper uptake defects of a strain lacking PIC2. When expressed in Lactococcus lactis, Mrs3 mediated copper and iron import. Finally, a PIC2 and MRS3 double mutant prevented the copper-dependent activation of a heterologously expressed copper sensor in the mitochondrial intermembrane space. Taken together, these data support a role for the iron transporter Mrs3 in copper import into the mitochondrial matrix.
引用
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页数:9
相关论文
共 40 条
[1]   Three conserved histidine residues contribute to mitochondrial iron transport through mitoferrins [J].
Brazzolotto, Xavier ;
Pierrel, Fabien ;
Pelosi, Ludovic .
BIOCHEMICAL JOURNAL, 2014, 460 :79-89
[2]   Yeast contain a non-proteinaceous pool of copper in the mitochondrial matrix [J].
Cobine, PA ;
Ojeda, LD ;
Rigby, KM ;
Winge, DR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (14) :14447-14455
[3]   Mitochondrial matrix copper complex used in metallation of cytochrome oxidase and superoxide dismutase [J].
Cobine, Paul A. ;
Pierrel, Fabien ;
Bestwick, Megan L. ;
Winge, Dennis R. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (48) :36552-36559
[4]  
DANCIS A, 1994, J BIOL CHEM, V269, P25660
[5]   A Targetable Fluorescent Sensor Reveals That Copper-Deficient SCO1 and SCO2 Patient Cells Prioritize Mitochondrial Copper Homeostasis [J].
Dodani, Sheel C. ;
Leary, Scot C. ;
Cobine, Paul A. ;
Winge, Dennis R. ;
Chang, Christopher J. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (22) :8606-8616
[6]   Cytochrome c oxidase biogenesis:: New levels of regulation [J].
Fontanesi, Flavia ;
Soto, Ileana C. ;
Barrientos, Antoni .
IUBMB LIFE, 2008, 60 (09) :557-568
[7]   A chemical potentiator of copper-accumulation used to investigate the iron-regulons of Saccharomyces cerevisiae [J].
Foster, Andrew W. ;
Dainty, Samantha J. ;
Patterson, Carl J. ;
Pohl, Ehmke ;
Blackburn, Hannah ;
Wilson, Clare ;
Hess, Corinna R. ;
Rutherford, Julian C. ;
Quaranta, Laura ;
Corran, Andy ;
Robinson, Nigel J. .
MOLECULAR MICROBIOLOGY, 2014, 93 (02) :317-330
[8]   The mitochondrial carrier Rim2 co-imports pyrimidine nucleotides and iron [J].
Froschauer, Elisabeth M. ;
Rietzschel, Nicole ;
Hassler, Melanie R. ;
Binder, Markus ;
Schweyen, Rudolf J. ;
Lill, Roland ;
Muehlenhoff, Ulrich ;
Wiesenberger, Gerlinde .
BIOCHEMICAL JOURNAL, 2013, 455 :57-65
[9]   The yeast mitochondrial carrier proteins Mrs3p/Mrs4p mediate iron transport across the inner mitochondrial membrane [J].
Froschauer, Elisabeth M. ;
Schweyen, Rudolf J. ;
Wiesenberger, Gerlinde .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2009, 1788 (05) :1044-1050
[10]   STRUCTURE OF CYTOCHROME-C-OXIDASE, ENERGY GENERATOR OF AEROBIC LIFE [J].
GENNIS, R ;
FERGUSONMILLER, S .
SCIENCE, 1995, 269 (5227) :1063-1064