Mitochondrion-Specific Live-Cell Bioprobe Operated in a Fluorescence Turn-On Manner and a Well-Designed Photoactivatable Mechanism

被引：91
作者：
Gu, Xinggui [1 ,2 ,3 ,4 ,5 ]
Zhao, Engui [1 ,2 ,3 ,4 ,5 ]
Lam, Jacky W. Y. [1 ,2 ,3 ,4 ,5 ]
Peng, Qian [6 ]
Xie, Yujun [6 ]
Zhang, Yilin [2 ,3 ,4 ,5 ]
Wong, Kam Sing [2 ,3 ,4 ,5 ]
Sung, Herman H. Y. [2 ,3 ,4 ,5 ]
Williams, Ian D. [2 ,3 ,4 ,5 ]
Tang, Ben Zhong [1 ,2 ,3 ,4 ,5 ,7 ]
机构：
[1] HKUST Shenzhen Res Inst, 9 Yuexing 1st RD,South Area,Hitech Pk, Shenzhen 518057, Peoples R China
[2] Hong Kong Univ Sci & Technol, Dept Chem, Kowloon, Hong Kong, Peoples R China
[3] Hong Kong Univ Sci & Technol, Dept Phys, Kowloon, Hong Kong, Peoples R China
[4] Hong Kong Univ Sci & Technol, Inst Mol Funct Mat, Div Life Sci, Kowloon, Hong Kong, Peoples R China
[5] Hong Kong Univ Sci & Technol, Div Biomed Engn, Kowloon, Hong Kong, Peoples R China
[6] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Beijing 100190, Peoples R China
[7] S China Univ Technol, State Key Lab Luminescent Mat & Device, SCUT HKUST Joint Res Lab, Guangdong Innovat Res Team, Guangzhou 51640, Guangdong, Peoples R China
来源：
ADVANCED MATERIALS | 2015年 / 27卷 / 44期
关键词：
AGGREGATION-INDUCED EMISSION; INTRAMOLECULAR CHARGE-TRANSFER; FLUOROPHORES; PROTEINS; ACTIVATION; CHEMISTRY; DYES;
D O I：
10.1002/adma.201503751
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel mitochondrion-specific live-cell fluorescence turn-on photoactivatable bioprobe, named o-TPP3M, is designed and readily prepared, operating in a new photoactivatable mechanism based on a twisted intramolecular charge-transfer effect, the restriction of intramolecular rotations, and photocyclodehydrogenation. This bioprobe exhibits unique photoactivation behavior, a large signal-to-background ratio, and no cytotoxicity to living cells, providing a promising candidate for varied bioresearch.
引用
收藏
页码：7093 / 7100
页数：8
相关论文
共 46 条
  • [1] Convenient, benign and scalable synthesis of 2-and 4-substituted benzylpiperidines
    Agai, B
    Proszenyák, G
    Tárkányi, G
    Vida, L
    Faigl, F
    [J]. EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, 2004, 2004 (17) : 3623 - 3632
  • [2] Arai S., 1987, J CHEM SOC P1, V3, P481
  • [3] Rhodamines NN: A Novel Class of Caged Fluorescent Dyes
    Belov, Vladimir N.
    Wurm, Christian A.
    Boyarskiy, Vadim P.
    Jakobs, Stefan
    Hell, Stefan W.
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (20) : 3520 - 3523
  • [4] Imaging intracellular fluorescent proteins at nanometer resolution
    Betzig, Eric
    Patterson, George H.
    Sougrat, Rachid
    Lindwasser, O. Wolf
    Olenych, Scott
    Bonifacino, Juan S.
    Davidson, Michael W.
    Lippincott-Schwartz, Jennifer
    Hess, Harald F.
    [J]. SCIENCE, 2006, 313 (5793) : 1642 - 1645
  • [5] Ring-Closure and Isomerization Capabilities of Spiropyran-Derived Merocyanine Isomers
    Buback, Johannes
    Nuernberger, Patrick
    Kullmann, Martin
    Langhojer, Florian
    Schmidt, Ralf
    Wuerthner, Frank
    Brixner, Tobias
    [J]. JOURNAL OF PHYSICAL CHEMISTRY A, 2011, 115 (16) : 3924 - 3935
  • [6] Dempsey GT, 2011, NAT METHODS, V8, P1027, DOI [10.1038/NMETH.1768, 10.1038/nmeth.1768]
  • [7] Mitochondrial targeting of α-tocopheryl succinate enhances its pro-apoptotic efficacy: A new paradigm for effective cancer therapy
    Dong, Lan-Feng
    Jameson, Victoria J. A.
    Tilly, David
    Prochazka, Lubomir
    Rohlena, Jakub
    Valis, Karel
    Truksa, Jaroslav
    Zobalova, Renata
    Mandavian, Elahe
    Kluckova, Katarina
    Stantic, Marina
    Stursa, Jan
    Freeman, Ruth
    Witting, Paul K.
    Norberg, Erik
    Goodwin, Jacob
    Salvatore, Brian A.
    Novotna, Jana
    Turanek, Jaroslav
    Ledvina, Miroslav
    Hozak, Pavel
    Zhivotovsky, Boris
    Coster, Mark J.
    Ralph, Stephen J.
    Smith, Robin A. J.
    Neuzil, Jiri
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 2011, 50 (11) : 1546 - 1555
  • [8] Photoactivatable fluorescein derivatives with azidomethyl caging groups for tracing oligonucleotides in living human cells
    Furukawa, Kazuhiro
    Abe, Hiroshi
    Tsuneda, Satoshi
    Ito, Yoshihiro
    [J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2010, 8 (10) : 2309 - 2311
  • [9] Structural changes accompanying intramolecular electron transfer: Focus on twisted intramolecular charge-transfer states and structures
    Grabowski, ZR
    Rotkiewicz, K
    Rettig, W
    [J]. CHEMICAL REVIEWS, 2003, 103 (10) : 3899 - 4031
  • [10] Aggregation-Induced Emission in the Crystals of 9,10-Distyrylanthracene Derivatives: The Essential Role of Restricted Intramolecular Torsion
    He, Jiating
    Xu, Bin
    Chen, Feipeng
    Xia, Haijian
    Li, Kunpeng
    Ye, Ling
    Tian, Wenjing
    [J]. JOURNAL OF PHYSICAL CHEMISTRY C, 2009, 113 (22) : 9892 - 9899
12345