Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model

被引:53
作者
Sigar, Ira M. [1 ]
Schripsema, Justin H. [1 ]
Wang, Yibing [2 ]
Clarke, Ian N. [2 ]
Cutcliffe, Lesley T. [2 ]
Seth-Smith, Helena M. B. [3 ]
Thomson, Nicholas R. [3 ]
Bjartling, Carina [4 ]
Unemo, Magnus [5 ]
Persson, Kenneth [6 ]
Ramsey, Kyle H. [1 ]
机构
[1] Midwestern Univ, Chicago Coll Osteopath Med, Dept Microbiol & Immunol, Downers Grove, IL 60516 USA
[2] Univ Southampton, Dept Clin & Expt Sci, Mol Microbiol Grp, Fac Med, Southampton, Hants, England
[3] Wellcome Trust Sanger Inst, Hinxton, Cambs, England
[4] Malmo Univ Hosp, Dept Obstet & Gynecol, Malmo, Sweden
[5] Orebro Univ Hosp, Dept Lab Med, Orebro, Sweden
[6] Malmo Univ Hosp, Dept Lab Med, Malmo, Sweden
基金
英国惠康基金;
关键词
Chlamydia; plasmid; mouse; virulence; infection; genome; TRANSCRIPTIONAL REGULATOR; GENITAL-INFECTION; MICE; VARIANT; STRAIN; DIVERSITY; PROTECTS; SEQUENCE; ARTEMIS; SWEDISH;
D O I
10.1111/2049-632X.12086
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C.trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as measured by weight change) when compared to the wild-type isolate. Further, differences in infectivity, but not in virulence were observed in a C.trachomatis, serovar E isolate with a deletion within the plasmid coding sequence 1 when compared to a serovar E isolate with no mutations in the plasmid. We conclude that plasmid loss reduces virulence and infectivity in this mouse model. These findings further support a role for the chlamydial plasmid in infectivity and virulence in vivo.
引用
收藏
页码:61 / 69
页数:9
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