Characterization and Identification of Dityrosine Cross-Linked Peptides Using Tandem Mass Spectrometry

被引:70
作者
Mukherjee, Soumya [1 ,2 ]
Kapp, Eugene A. [1 ,3 ]
Lothian, Amber [1 ,3 ]
Roberts, Anne M. [1 ,3 ]
Vasil'ev, Yury V. [4 ]
Boughton, Berin A. [5 ]
Barnham, Kevin J. [1 ,6 ]
Kok, W. Mei [6 ,7 ]
Hutton, Craig A. [6 ,7 ]
Masters, Colin L. [1 ,3 ]
Bush, Ashley I. [1 ,3 ]
Beckmann, Joseph S. [4 ]
Dey, Somdatta Ghosh [2 ]
Roberts, Blaine R. [1 ,3 ]
机构
[1] Univ Melbourne, Florey Inst Neurosci & Mental Hlth, 30 Royal Parade, Parkville, Vic 3052, Australia
[2] Indian Assoc Cultivat Sci, Dept Inorgan Chem, Kolkata 700032, India
[3] Cooperat Res Ctr Mental Hlth, Parkville, Vic 3052, Australia
[4] Oregon State Univ, Linus Pauling Inst, Dept Biochem & Biophys, Corvallis, OR 97331 USA
[5] Univ Melbourne, Metabol Australia, Sch Biosci, Parkville, Vic 3052, Australia
[6] Univ Melbourne, Mol Sci & Biotechnol Inst Bio21, Parkville, Vic 3052, Australia
[7] Univ Melbourne, Sch Chem, Parkville, Vic 3052, Australia
基金
澳大利亚研究理事会;
关键词
TYROSINE OXIDATION-PRODUCTS; ELECTRON-TRANSFER DISSOCIATION; HUMAN CEREBROSPINAL-FLUID; PARKINSONS-DISEASE; ALPHA-SYNUCLEIN; PROTEIN IDENTIFICATION; AMYLOID-BETA; O-TYROSINE; HEMOGLOBIN; QUANTIFICATION;
D O I
10.1021/acs.analchem.7b00941
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The use of mass spectrometry coupled with Chemical cross-linking of proteins has become a powerful tool for proteins structure and interactions studies. Unlike structural analysis of proteins using chemical reagents specific for lysine or Cysteine residues, identification of gas-phase fragmentation patterns of endogenus dityrosine Cross-linked peptides have not been investigated. Dityrosine cross-linking in proteins and peptides are clinical markers of oxidative stress,, aging, and neurodegenerative diseases including Alzheimer's disease and Parkinson's disease. In this study, we investigated and characterized the fragmentation pattern of a synthetically prepared dityrosine cross-linked driller of A beta(1-16), using ESI tandem mass spectrometry. We then detailed the fragmentation pattern of dityrosine cross-linked A beta(1-16), using collision induced dissociation (CID), higher-energy collision induced dissociation (HCD), electron transfer dissociation (ETD), and electron capture dissociation (ECD). Application of these generic fragmentation rules of dityrosine cross-linked peptides allowed for the identification of dityrosine cross-links in peptides of A beta and alpha-synuclein generated in vitro by enzymatic peroxidation. We report, for the first time, the dityrosine cross-linked residues in human hemoglobin and alpha-synuclein under oxidative conditions. Together these tools open up the potential for automated analysis of this naturally occurring post-translation modification in neurodegenerative diseases as well as other pathological conditions.
引用
收藏
页码:6137 / 6146
页数:10
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