Ribosome-display technology: applications for directed evolution of functional proteins

被引：31

作者：

Yan, Xianghua

Xu, Zirong ^{[1
]}

机构：

[1] Zhejiang Univ, Antibody Engn Ctr, Key Lab Anim Med Nutr, Minist Educ,Feed Sci Inst, Hangzhou 310029, Peoples R China

[2] Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan 430070, Peoples R China

来源：

DRUG DISCOVERY TODAY | 2006年 / 11卷 / 19-20期

关键词：

D O I：

10.1016/j.drudis.2006.08.012

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In vitro display technologies, especially ribosome display, are valuable tools for many applications. In this paper, ribosome display technology and its applications for directed evolution of functional proteins will be reviewed. Ribosome display has great potential for directed evolution of protein stability and affinity, the generation of high-quality libraries by in vitro preselection, the selection of enzymatic activities, and the display of cDNA and random-peptide libraries. Ribosome display is carried out fully in vitro, which overcomes some of the limitations of cell-based display systems. We anticipate that ribosome display will have a great impact on applications in biotechnology, medicine and proteomics.

引用

页码：911 / 916

页数：6

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