Oral vaccination of mice against Helicobacter pylori with recombinant Lactococcus lactis expressing urease subunit B

被引:31
作者
Gu, Qing [1 ]
Song, Dafeng [1 ]
Zhu, Muyuan [2 ]
机构
[1] Zhejiang Gongshang Univ, Dept Biotechnol, Hangzhou 310035, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Life Sci, Inst Cell Biol & Genet, Hangzhou 310003, Zhejiang, Peoples R China
来源
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2009年 / 56卷 / 03期
基金
中国国家自然科学基金;
关键词
Helicobacter pylori; UreB; Lactococcus lactis; vaccine; TOXIN FRAGMENT-C; ESCHERICHIA-COLI; ACID BACTERIA; MUCOSAL; DELIVERY; LIVE; IMMUNOGENICITY; ANTIGENS; TETANUS; PROTECTION;
D O I
10.1111/j.1574-695X.2009.00566.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
To determine whether a protective immune response could be elicited by oral delivery of a recombinant live bacterial vaccine, Helicobacter pylori urease subunit B (UreB) was expressed for extracellular expression in food-grade bacterium Lactococcus lactis. The UreB-producing strains were then administered orally to mice, and the immune response to UreB was examined. Orally vaccinated mice produced a significant UreB-specific serum immunoglobulin G (IgG) response. Specific anti-UreB IgA responses could be detected in the feces of mice immunized with the secreting lactococcal strain. Mice vaccinated orally were significantly protected against gastric Helicobacter infection following a challenge with H. pylori strain SS1. In conclusion, mucosal vaccination with L. lactis expressing UreB produced serum IgG and UreB-specific fecal IgA, and prevented gastric infection with H. pylori.
引用
收藏
页码:197 / 203
页数:7
相关论文
共 38 条
[1]   ON THE SAFETY OF LACTIC-ACID BACTERIA FROM FOOD [J].
ADAMS, MR ;
MARTEAU, P .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1995, 27 (2-3) :263-264
[2]   Lactococcus lactis-expressing listeriolysin O (LLO) provides protection and specific CD8+ T cells against Listeria monocytogenes in the murine infection model [J].
Bahey-El-Din, Mohammed ;
Casey, Pat G. ;
Griffin, Brendan T. ;
Gahan, Cormac G. M. .
VACCINE, 2008, 26 (41) :5304-5314
[3]   Helicobacter pylori:: the challenge in therapy [J].
Bazzoli, F ;
Pozzato, P ;
Rokkast, T .
HELICOBACTER, 2002, 7 :43-49
[4]   The design of vaccines against Helicobacter pylori and their development [J].
Del Giudice, G ;
Covacci, A ;
Telford, JL ;
Montecucco, C ;
Rappuoli, R .
ANNUAL REVIEW OF IMMUNOLOGY, 2001, 19 :523-563
[5]   Ability of Lactococcus lactis to export viral capsid antigens:: a crucial step for development of live vaccines [J].
Dieye, Y ;
Hoekman, AJW ;
Clier, F ;
Juillard, V ;
Boot, HJ ;
Piard, JC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (12) :7281-7288
[6]   Bovine rotavirus nonstructural protein 4 produced by Lactococcus lactis is antigenic and immunogenic [J].
Enouf, V ;
Langella, P ;
Commissaire, J ;
Cohen, J ;
Corthier, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (04) :1423-1428
[7]   RECOMBINANT ANTIGENS PREPARED FROM THE UREASE SUBUNITS OF HELICOBACTER SPP - EVIDENCE OF PROTECTION IN A MOUSE MODEL OF GASTRIC INFECTION [J].
FERRERO, RL ;
THIBERGE, JM ;
HUERRE, M ;
LABIGNE, A .
INFECTION AND IMMUNITY, 1994, 62 (11) :4981-4989
[8]   SIMULTANEOUS VISUALIZATION OF HELICOBACTER-PYLORI AND GASTRIC MORPHOLOGY - A NEW STAIN [J].
GENTA, RM ;
ROBASON, GO ;
GRAHAM, DY .
HUMAN PATHOLOGY, 1994, 25 (03) :221-226
[9]   Helicobacter pylori urease is a potent stimulus of mononuclear phagocyte activation and inflammatory cytokine production [J].
Harris, PR ;
Mobley, HLT ;
PerezPerez, GI ;
Blaser, MJ ;
Smith, PD .
GASTROENTEROLOGY, 1996, 111 (02) :419-425
[10]   HIGH-FREQUENCY TRANSFORMATION, BY ELECTROPORATION, OF LACTOCOCCUS-LACTIS SUBSP CREMORIS GROWN WITH GLYCINE IN OSMOTICALLY STABILIZED MEDIA [J].
HOLO, H ;
NES, IF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (12) :3119-3123