共 38 条
Oral vaccination of mice against Helicobacter pylori with recombinant Lactococcus lactis expressing urease subunit B
被引:31
作者:
Gu, Qing
[1
]
Song, Dafeng
[1
]
Zhu, Muyuan
[2
]
机构:
[1] Zhejiang Gongshang Univ, Dept Biotechnol, Hangzhou 310035, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Life Sci, Inst Cell Biol & Genet, Hangzhou 310003, Zhejiang, Peoples R China
来源:
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
|
2009年
/
56卷
/
03期
基金:
中国国家自然科学基金;
关键词:
Helicobacter pylori;
UreB;
Lactococcus lactis;
vaccine;
TOXIN FRAGMENT-C;
ESCHERICHIA-COLI;
ACID BACTERIA;
MUCOSAL;
DELIVERY;
LIVE;
IMMUNOGENICITY;
ANTIGENS;
TETANUS;
PROTECTION;
D O I:
10.1111/j.1574-695X.2009.00566.x
中图分类号:
R392 [医学免疫学];
Q939.91 [免疫学];
学科分类号:
100102 ;
摘要:
To determine whether a protective immune response could be elicited by oral delivery of a recombinant live bacterial vaccine, Helicobacter pylori urease subunit B (UreB) was expressed for extracellular expression in food-grade bacterium Lactococcus lactis. The UreB-producing strains were then administered orally to mice, and the immune response to UreB was examined. Orally vaccinated mice produced a significant UreB-specific serum immunoglobulin G (IgG) response. Specific anti-UreB IgA responses could be detected in the feces of mice immunized with the secreting lactococcal strain. Mice vaccinated orally were significantly protected against gastric Helicobacter infection following a challenge with H. pylori strain SS1. In conclusion, mucosal vaccination with L. lactis expressing UreB produced serum IgG and UreB-specific fecal IgA, and prevented gastric infection with H. pylori.
引用
收藏
页码:197 / 203
页数:7
相关论文