Cell detection and tracking in lab-on-a-chip devices by image processing

被引：5

作者：

Lizanets, Danylo ^{[1
]}

Walczak, Rafal ^{[1
]}

机构：

[1] Wroclaw Univ Sci & Technol, Fac Microsyst Elect & Photon, Janiszewskiego 11-17, PL-50372 Wroclaw, Poland

来源：

OPTICA APPLICATA | 2018年 / 48卷 / 01期

关键词：

image processing; lab-on-a-chip; computer vision; bioanalysis; SINGLE CELLS; DROPLETS;

D O I：

10.5277/oa180102

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

This paper is devoted to the usage of image processing techniques in lab-on-a-chip devices for a cell detection and tracking. Advantages and disadvantages of image processing in data acquisition from lab-on-a-chip are described. A modified multiparametric object tracking method is presented. The method was tested on the lab-on-a-chip setup with living euglena and has shown plausible results for video with a low frame rate.

引用

页码：15 / 24

页数：10

共 15 条

[1]

Falconnet D, 2011, LAB CHIP, V11, P466, DOI [10.1039/c0lc00228c, 10.1039/c01c00228c]

[2] Target identification by image analysis [J].

Fetz, V. ;

Prochnow, H. ;

Broenstrup, M. ;

Sasse, F. .

NATURAL PRODUCT REPORTS, 2016, 33 (05) :655-667

[3] A multiscale algorithm for nuclei extraction in pap smear images [J].

Garcia-Gonzalez, Dibet ;

Garcia-Silvente, Miguel ;

Aguirre, Eugenio .

EXPERT SYSTEMS WITH APPLICATIONS, 2016, 64 :512-522

[4] Robust single-particle tracking in live-cell time-lapse sequences [J].

Jaqaman, Khuloud ;

Loerke, Dinah ;

Mettlen, Marcel ;

Kuwata, Hirotaka ;

Grinstein, Sergio ;

Schmid, Sandra L. ;

Danuser, Gaudenz .

NATURE METHODS, 2008, 5 (08) :695-702

[5] Automated analysis of dynamic behavior of single cells in picoliter droplets [J].

Khorshidi, Mohammad Ali ;

Rajeswari, Prem Kumar Periyannan ;

Wahlby, Carolina ;

Joensson, Haakan N. ;

Svahn, Helene Andersson .

LAB ON A CHIP, 2014, 14 (05) :931-937

[6] Mechanism of action-based classification of antibiotics using high-content bacterial image analysis [J].

Peach, Kelly C. ;

Bray, Walter M. ;

Winslow, Dustin ;

Linington, Peter F. ;

Linington, Roger G. .

MOLECULAR BIOSYSTEMS, 2013, 9 (07) :1837-1848

[7] Single-cell bacteria growth monitoring by automated DEP-facilitated image analysis [J].

Peitz, Ingmar ;

van Leeuwen, Rien .

LAB ON A CHIP, 2010, 10 (21) :2944-2951

[8] An integrated image analysis platform to quantify signal transduction in single cells [J].

Pelet, Serge ;

Dechant, Reinhard ;

Lee, Sung Sik ;

van Drogen, Frank ;

Peter, Matthias .

INTEGRATIVE BIOLOGY, 2012, 4 (10) :1274-1282

[9]

Podwin Agnieszka, 2016, Elektronika, V57, P85, DOI 10.15199/13.2016.10.21

[10] An integrated one-step system to extract, analyze and annotate all relevant information from image-based cell screening of chemical libraries [J].

Rabal, Obdulia ;

Link, Wolfgang ;

Serelde, Beatriz G. ;

Bischoff, James R. ;

Oyarzabal, Julen .

MOLECULAR BIOSYSTEMS, 2010, 6 (04) :711-720

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