Frequently asked questions about in vivo chlorophyll fluorescence: practical issues

被引：618

作者：

Kalaji, Hazem M. ^{[1
]}

Schansker, Gert

Ladle, Richard J. ^{[2
]}

Goltsev, Vasilij ^{[3
]}

Bosa, Karolina ^{[4
]}

Allakhverdiev, Suleyman I. ^{[5
,6
]}

Brestic, Marian ^{[7
]}

Bussotti, Filippo ^{[8
]}

Calatayud, Angeles ^{[9
]}

Dabrowski, Piotr ^{[10
]}

Elsheery, Nabil I. ^{[11
]}

Ferroni, Lorenzo ^{[12
]}

Guidi, Lucia ^{[13
]}

Hogewoning, Sander W. ^{[14
]}

Jajoo, Anjana ^{[15
]}

Misra, Amarendra N. ^{[16
]}

Nebauer, Sergio G. ^{[17
]}

Pancaldi, Simonetta ^{[12
]}

Penella, Consuelo ^{[9
]}

Poli, DorothyBelle ^{[18
]}

Pollastrini, Martina ^{[8
]}

Romanowska-Duda, Zdzislawa B. ^{[19
]}

Rutkowska, Beata ^{[20
]}

Serodio, Joao ^{[21
]}

Suresh, Kancherla ^{[22
]}

Szulc, Wieslaw ^{[20
]}

Tambussi, Eduardo ^{[23
]}

Yanniccari, Marcos ^{[23
]}

Zivcak, Marek ^{[7
]}

机构：

[1] Warsaw Univ Life Sci SGGW, Fac Agr & Biol, Dept Plant Physiol, Nowoursynowska 159, PL-02776 Warsaw, Poland

[2] Univ Fed Alagoas, Inst Biol & Hlth Sci, Maceio, AL, Brazil

[3] St Kliment Ohridski Univ Sofia, Dept Biophys & Radiobiol, Fac Biol, Sofia 1164, Bulgaria

[4] Warsaw Univ Life Sci SGGW, Dept Pomol, Fac Hort Biotechnol & Landscape Architecture, PL-02776 Warsaw, Poland

[5] Russian Acad Sci, Inst Plant Physiol, Moscow 127276, Russia

[6] Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142290, Moscow Region, Russia

[7] Slovak Univ Agr, Dept Plant Physiol, Nitra 94976, Slovakia

[8] Univ Florence, Dept Agri Food Prod & Environm Sci DISPAA, I-50144 Florence, Italy

[9] Inst Valenciano Invest Agr, Dept Hort, Valencia 46113, Spain

[10] Warsaw Univ Life Sci SGGW, Fac Civil & Environm Engn, Dept Environm Improvement, PL-02776 Warsaw, Poland

[11] Tanta Univ, Fac Agr, Dept Agr Bot, Tanta, Egypt

[12] Univ Ferrara, Dept Life Sci & Biotechnol, I-44121 Ferrara, Italy

[13] Dept Agr Food & Environm, I-56124 Pisa, Italy

[14] Plant Lighting BV, NL-3981 PC Bunnik, Netherlands

[15] Devi Ahilya Univ, Sch Life Sci, Indore 452001, Madhya Pradesh, India

[16] Cent Univ Jharkhand, Ctr Life Sci, Ranchi 835205, Bihar, India

[17] Univ Politecn Valencia, Dept Prod Vegetal, Valencia 46022, Spain

[18] Roanoke Coll, Dept Biol, Salem, VA 24153 USA

[19] Univ Lodz, Dept Ecophysiol & Plant Dev, PL-90237 Lodz, Poland

[20] Warsaw Univ Life Sci SGGW, Fac Agr & Biol, Dept Agr Chem, PL-02776 Warsaw, Poland

[21] Univ Aveiro, Dept Biol, CESAM Ctr Estudos Ambiente & Mar, P-3810193 Aveiro, Portugal

[22] Directorate Oil Palm Res, Pedavegi 534450, Andhra Pradesh, India

[23] INFIVE Univ Nacl La Plata, Inst Plant Physiol, CONICET, RA-327 La Plata, Buenos Aires, Argentina

来源：

PHOTOSYNTHESIS RESEARCH | 2014年 / 122卷 / 02期

关键词：

Chlorophyll a fluorescence; Fluorescence imaging; Complementary techniques; Frequently asked questions; Plant stress monitoring; Photosynthesis; PHOTOSYNTHETIC ELECTRON-TRANSPORT; LIGHT-HARVESTING COMPLEXES; NONPHOTOCHEMICAL ENERGY-DISSIPATION; STRONG CONTINUOUS ILLUMINATION; INDUCED ABSORBENCY CHANGES; PHOTOSYSTEM-II COMPLEXES; OXYGEN-EVOLVING COMPLEX; A FLUORESCENCE; THYLAKOID MEMBRANES; EXCITATION-ENERGY;

D O I：

10.1007/s11120-014-0024-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.

引用

页码：121 / 158

页数：38

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